Vito C C, Fox T O
Brain Res. 1981 Aug;254(1):97-110. doi: 10.1016/0165-3806(81)90062-6.
We provide biochemical evidence that specific androgen- and estrogen-binding macromolecules are present in rat hypothalamus-preoptic area throughout the "critical period' of brain sexual differentiation. Macromolecules with the properties of putative androgen and estrogen receptors are present in cytosolic extracts of embryonic neonatal, prepubertal rat hypothalamus-preoptic area and other brain regions. Both the androgen and estrogen receptors in perinatal rat brain are qualitatively similar to those in adult brain by virtue of their high affinity and limited capacity for hormone, specificity of hormone-binding, ability to adhere to DNA, differential behavior during DNA-cellulose affinity chromatography, behavior during velocity sedimentation, and tissue specificity. As early as 7 days before birth, both androgen and estrogen receptors are detectable in hypothalamus-preoptic area and other brain regions. Both receptors are more abundant in the hypothalamus-preoptic area relative to other regions. The concentrations of both receptors in hypothalamus-preoptic area increases as a function of age, although the relative rate of appearance of each receptor is distinctive: estrogen receptor concentrations increase approximately 6-fold during the last week of gestation, approximately adult-like levels at birth; androgen receptor concentrations increase slightly during late gestation, gradually rising to adult-like levels a few weeks after birth. The ontogeny profiles of both receptors from rat hypothalamus-preoptic area are compared with those from mouse. The presence of sex hormone receptors in perinatal rodent brain is discussed in the context of the hormonal milieu of perinatal rodent brain, and its effects on sexual differentiation of neural organization. The coincident appearance of receptors and post-mitotic neurons in prenatal rodent brain may indicate that sex hormones effect the early differentiation of these neurons.
我们提供了生化证据，表明在大脑性分化的“关键时期”，大鼠下丘脑-视前区存在特定的雄激素和雌激素结合大分子。具有假定雄激素和雌激素受体特性的大分子存在于胚胎期、新生期、青春期前大鼠下丘脑-视前区及其他脑区的胞质提取物中。围产期大鼠脑中的雄激素和雌激素受体在性质上与成年脑相似，这体现在它们对激素的高亲和力和有限容量、激素结合特异性、黏附于DNA的能力、在DNA-纤维素亲和层析中的差异行为、速度沉降行为以及组织特异性上。早在出生前7天，下丘脑-视前区及其他脑区就能检测到雄激素和雌激素受体。相对于其他区域，这两种受体在下丘脑-视前区更为丰富。下丘脑-视前区中这两种受体的浓度随年龄增长而增加，尽管每种受体出现的相对速率有所不同：雌激素受体浓度在妊娠最后一周增加约6倍，出生时达到近似成年水平；雄激素受体浓度在妊娠后期略有增加，出生后几周逐渐上升至成年水平。将大鼠下丘脑-视前区这两种受体的个体发育情况与小鼠的进行了比较。在围产期啮齿动物脑的激素环境及其对神经组织性分化的影响背景下，讨论了围产期啮齿动物脑中性激素受体的存在情况。产前啮齿动物脑中受体与有丝分裂后神经元的同时出现可能表明性激素影响这些神经元的早期分化。
