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干细胞来源的卵巢类器官构建的研究进展

Research advances in the construction of stem cell-derived ovarian organoids.

作者信息

Zhang Tianyue, Zhang Mengtong, Zhang Sichen, Wang Shaowei

机构信息

Department of Gynecology and Obstetrics, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, 100730, People's Republic of China.

Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.

出版信息

Stem Cell Res Ther. 2024 Dec 31;15(1):505. doi: 10.1186/s13287-024-04122-3.

DOI:10.1186/s13287-024-04122-3
PMID:39736770
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11687137/
Abstract

Ovarian organoids are essential in female reproductive medicine, enhancing our understanding of ovarian diseases and improving treatments, which benefits women's health. Constructing ovarian organoids involves two main processes: differentiating induced pluripotent stem cells (iPSCs) into germ and ovarian somatic cells to restore ovarian function and using extracellular matrix (ECM) to create a suitable ovarian microenvironment and scaffold. Although the technology is still in its early stages, future advancements will likely involve integrating high-throughput analysis, 3D-printed scaffolds, and efficient iPSC induction, driving progress in reproductive and regenerative medicine.

摘要

卵巢类器官在女性生殖医学中至关重要，有助于我们加深对卵巢疾病的理解并改善治疗方法，从而造福女性健康。构建卵巢类器官涉及两个主要过程：将诱导多能干细胞（iPSC）分化为生殖细胞和卵巢体细胞以恢复卵巢功能，以及使用细胞外基质（ECM）创建合适的卵巢微环境和支架。尽管该技术仍处于早期阶段，但未来的进展可能会涉及整合高通量分析、3D打印支架和高效的iPSC诱导，推动生殖医学和再生医学的发展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e85b/11687137/fae55cd9cb9b/13287_2024_4122_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e85b/11687137/d60fe23c3817/13287_2024_4122_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e85b/11687137/276646df9cfa/13287_2024_4122_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e85b/11687137/a6c0f53764de/13287_2024_4122_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e85b/11687137/fae55cd9cb9b/13287_2024_4122_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e85b/11687137/d60fe23c3817/13287_2024_4122_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e85b/11687137/276646df9cfa/13287_2024_4122_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e85b/11687137/a6c0f53764de/13287_2024_4122_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e85b/11687137/fae55cd9cb9b/13287_2024_4122_Fig4_HTML.jpg

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本文引用的文献

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Genes Cells. 2024 Oct;29(10):797-807. doi: 10.1111/gtc.13152. Epub 2024 Aug 9.
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Research progress on the application of organoids in gynecological tumors.类器官在妇科肿瘤中的应用研究进展
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Generation of Tailored Extracellular Matrix Hydrogels for the Study of In Vitro Folliculogenesis in Response to Matrisome-Dependent Biochemical Cues.
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Liquid nitrogen improves the decellularization effectiveness of whole-ovary.液氮提高了整个卵巢脱细胞的效果。
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Optimizing Decellularization of Bovine Ovarian Tissue: Toward a Transplantable Artificial Ovary Scaffold with Minimized Residual Toxicity and Preserved Extracellular Matrix Morphology.优化牛卵巢组织脱细胞处理：制备具有最小残留毒性和保留细胞外基质形态的可移植人工卵巢支架。
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