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病毒正义链、反义链和发夹结构诱导的对甜菜曲顶病毒和伊朗甜菜曲顶病毒的抗性

Resistance Induced by Viral Sense, Anti-sense, and Hairpin Constructs Against Beet Curly Top Virus and Beet Curly Top Iran Virus.

作者信息

Montazeri Razieh, Malboobi Mohammad Ali, Shams-Bakhsh Masoud

机构信息

Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

National Institute of Genetic Engineering and Biotechnology, Department of Plant Biotechnology, Tehran, Iran.

出版信息

Iran J Biotechnol. 2024 Jul 1;22(3):e3831. doi: 10.30498/ijb.2024.425159.3831. eCollection 2024 Jul.

DOI:10.30498/ijb.2024.425159.3831
PMID:39737209
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11682523/
Abstract

BACKGROUND

RNA silencing-based antiviral breeding is a promising strategy for developing virus-resistant plants.

OBJECTIVES

This study employed viral sense, anti-sense, and hairpin constructs to induce resistance against beet curly top virus (BCTV) and beet curly top Iran virus (BCTIV).

MATERIALS AND METHODS

For this purpose, a 120-bp conserved sequence of Rep- and C2-BCTV and a 222-bp conserved sequence of CP-, Reg-, and MP-BCTIV were selected for construct production. The efficiency of constructs was investigated in transient expression in and sugar beet plants and stable expression in .

RESULTS

In transient expression, all designed constructs induced effective resistance to BCTV and BCTIV; the hairpin constructs were more effective against both viruses. The stability of the achieved resistance by hairpin constructs was also confirmed in the T1 generation of transgenic plants.

CONCLUSIONS

This study showed that employing conserved coding sequences of BCTVs leads to effective resistance against BCTVs infection. The lack of protein production from transgene and degradation of its transcript due to the gene silencing mechanism makes this method safe for biosecurity. In stable transformation, the inheritance of induced resistance against BCTVs was confirmed in the T1 generation. These advantages make this mechanism commercially useful for the production of resistant plants to viruses.

摘要

背景

基于RNA沉默的抗病毒育种是培育抗病毒植物的一种有前景的策略。

目的

本研究采用病毒正义、反义及发夹结构来诱导对甜菜曲顶病毒（BCTV）和伊朗甜菜曲顶病毒（BCTIV）的抗性。

材料与方法

为此，选择Rep-和C2-BCTV的120bp保守序列以及CP-、Reg-和MP-BCTIV的222bp保守序列用于构建体的制备。在烟草和甜菜植株中研究构建体在瞬时表达中的效率以及在[此处原文缺失相关内容]中的稳定表达。

结果

在瞬时表达中，所有设计的构建体均诱导了对BCTV和BCTIV的有效抗性；发夹结构构建体对两种病毒更有效。在转基因植株的T1代中也证实了发夹结构构建体所获得抗性的稳定性。

结论

本研究表明，采用BCTVs的保守编码序列可有效抵抗BCTVs感染。由于基因沉默机制，转基因不产生蛋白质且其转录本降解，使得该方法对生物安全是安全的。在稳定转化中，在T1代中证实了对BCTVs诱导抗性的遗传。这些优点使得该机制在商业上可用于生产抗病毒植物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f48/11682523/4a54fd543408/IJB-22-e3831-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f48/11682523/676140f53c17/IJB-22-e3831-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f48/11682523/2b83c454a2c9/IJB-22-e3831-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f48/11682523/abb6bb01ef1a/IJB-22-e3831-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f48/11682523/f671c8a1cfe8/IJB-22-e3831-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f48/11682523/4a54fd543408/IJB-22-e3831-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f48/11682523/676140f53c17/IJB-22-e3831-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f48/11682523/2b83c454a2c9/IJB-22-e3831-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f48/11682523/abb6bb01ef1a/IJB-22-e3831-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f48/11682523/f671c8a1cfe8/IJB-22-e3831-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f48/11682523/4a54fd543408/IJB-22-e3831-g005.jpg

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Unusual outbreaks of curly top disease in processing tomato fields in northern California in 2021 and 2022 were caused by a rare strain of beet curly top virus and facilitated by extreme weather events.2021 年和 2022 年，加利福尼亚州北部加工番茄田发生了不寻常的卷叶病疫情，这是由一种罕见的甜菜曲顶病毒株引起的，极端天气事件也助长了这种病毒的传播。
Virology. 2024 Mar;591:109981. doi: 10.1016/j.virol.2024.109981. Epub 2024 Jan 4.
3
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