Barzegar-Bafrouei Raziyeh, Hajimohammadi Bahador, Zandi Hengameh, Eslami Gilda, Fallahzadeh Hossein
Department of Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Nutrition, Faculty of Health, Golestan University of Medical Sciences, Gorgan, Iran.
Iran J Microbiol. 2024 Dec;16(6):755-762. doi: 10.18502/ijm.v16i6.17248.
Salmonellosis is among the most common food-born infections, caused by spp. bacteria. Present study has investigated the frequency and antibiotic resistance pattern of spp. isolated from traditional dairy products and raw milk supplied in Yazd, Iran.
In a cross-sectional study, 350 samples of raw milk and traditional dairy products were randomly collected from July to September 2018. Following culturing the samples, isolates went through biochemical tests for phenotypic identification. Results were confirmed through PCR technique by targeting gene. Antimicrobial susceptibility test was conducted by means of disk diffusion method.
The rate of contamination with bacteria was 6.57% in all samples. The PCR assay of all isolates showed that 23 isolates (100%) carried the gene. No significant association between the frequency of spp. and types of dairy and their origin was reported (>0.05). The highest antibiotic resistance rate among the isolates belonged to tetracycline (34.8%) and the highest sensitivity was seen to imipenem, cefepime, and cefotaxime (each 91.3%).
According to our results there has been a rise in multiple drug resistance and contamination rate in traditional dairy products in Yazd province.
沙门氏菌病是最常见的食源性感染之一，由沙门氏菌属细菌引起。本研究调查了从伊朗亚兹德供应的传统乳制品和生牛奶中分离出的沙门氏菌属的频率和抗生素耐药模式。
在一项横断面研究中，于2018年7月至9月随机收集了350份生牛奶和传统乳制品样本。对样本进行培养后，分离株通过生化试验进行表型鉴定。通过靶向invA基因的PCR技术对结果进行确认。采用纸片扩散法进行药敏试验。
所有样本中沙门氏菌属细菌的污染率为6.57%。所有分离株的PCR检测显示，23株（100%）携带invA基因。未报告沙门氏菌属频率与乳制品类型及其来源之间存在显著关联（P>0.05）。分离株中最高的抗生素耐药率属于四环素（34.8%），对亚胺培南、头孢吡肟和头孢噻肟的敏感性最高（均为91.3%）。
根据我们的结果，亚兹德省传统乳制品中的多重耐药性和污染率有所上升。
