Zhang Luzheng, Chen Shuyang, Ning Mengling, Guo Suxiang, Wen Dezhong, Wang Heng, Sun Yujin, Yang Guangdong, Wang Yuehong, Xue Song
Department of Cardiovascular Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P. R. China.
Department of Pulmonary and Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, P. R. China.
ACS Appl Bio Mater. 2025 Jan 20;8(1):688-703. doi: 10.1021/acsabm.4c01549. Epub 2024 Dec 31.
Abdominal aortic aneurysm (AAA) is a cardiovascular disease with potentially fatal consequences, yet effective therapies to prevent its progression remain unavailable. Oxidative stress is associated with AAA development. Carbon dots have reactive oxygen species-scavenging activity, while green tea extract exhibits robust antioxidant properties. However, the potential of green tea derived carbon dots in mitigating AAA progression has not been fully elucidated. In this study, tea polyphenol carbon dots (TP-CDs) were synthesized via hydrothermal methods and characterized for their antioxidant properties. The antioxidant effects of TP-CDs were evaluated, and TP-CDs' impact on phenotypic transformation, oxidative stress, apoptosis and ferroptosis was investigated comprehensively in an Ang II-induced AAA model, employing techniques such as Western blotting, flow cytometry, and immunohistochemistry. The results revealed that TP-CDs effectively alleviated oxidative stress induced by Ang II stimulation, thereby inhibiting phenotypic transformation, apoptosis, and ferroptosis . Furthermore, treatment with TP-CDs significantly attenuated AAA progression in a mouse AAA model. Overall, these findings demonstrate that TP-CDs reduced reactive oxygen species levels in the microenvironment and alleviated the progression of AAA, offering a promising therapeutic strategy for this condition.
腹主动脉瘤（AAA）是一种具有潜在致命后果的心血管疾病，但目前仍缺乏有效的预防其进展的治疗方法。氧化应激与AAA的发展有关。碳点具有清除活性氧的能力，而绿茶提取物具有强大的抗氧化特性。然而，绿茶衍生碳点在减轻AAA进展方面的潜力尚未完全阐明。在本研究中，通过水热法合成了茶多酚碳点（TP-CDs），并对其抗氧化性能进行了表征。评估了TP-CDs的抗氧化作用，并在血管紧张素II诱导的AAA模型中，采用蛋白质免疫印迹法、流式细胞术和免疫组织化学等技术，全面研究了TP-CDs对表型转化、氧化应激、细胞凋亡和铁死亡的影响。结果表明，TP-CDs有效减轻了血管紧张素II刺激诱导的氧化应激，从而抑制了表型转化、细胞凋亡和铁死亡。此外，在小鼠AAA模型中，TP-CDs治疗显著减轻了AAA的进展。总体而言，这些发现表明，TP-CDs降低了微环境中的活性氧水平，减轻了AAA的进展，为这种疾病提供了一种有前景的治疗策略。
