Ali Zainab Haider, Abdulazeem Lubna, Kadhim Wurood Alwan, Kzar Mazin H, Al-Sareji Osamah J
Department of Biology, College of Science for Women, University of Babylon, Babylon, Iraq.
DNA Research Center, University of Babylon, Babylon, Iraq.
Sci Rep. 2024 Dec 30;14(1):31593. doi: 10.1038/s41598-024-76090-w.
Ensuring everyone enjoys healthy lifestyles and well-being at all ages, Progress has been made in increasing access to clean water and sanitation facilities and reducing the spread of epidemics and diseases. The synthesis of nano-particles (NPs) by using microalgae is a new nanobiotechnology due to the use of the biomolecular (corona) of microalgae as a capping and reducing agent for NP creation. This investigation explores the capacity of a distinct indigenous microalgal strain to synthesize silver nano-particles (AgNPs), as well as its effectiveness against multi-drug resistant (MDR) bacteria and its ability to degrade Azo dye (Methyl Red) in wastewater. An extract of Spirulina platensis was obtained from a local source to synthesize silver nano-particles (AgNPs). The synthesized AgNPs were subsequently subjected to characterization utilizing several analytical methods, namely UV-visible spectroscopy, scanning electron microscopy (SEM), X-ray diffraction, and Fourier transform infrared spectroscopy (FTIR analysis). Subsequently, the disc diffusion method assessed their anti-bacterial efficacy against multi-drug resistant (MDR) bacteria and their ability to degrade Azo dye (Methyl Red) in wastewater. The nano-particles produced through biological synthesis exhibited a prominent peak in the UV-visible spectrum at a wavelength of 430 nm. Furthermore, these nano-particles were determined to possess a crystalline nature, with an average size of 28.72 nm and a distinctive star-like shape. The synthesized silver nano-particles (AgNPs) exhibited a dose-dependent anti-bacterial effect against some clinical bacterial isolates as multi-drug resistant (MDR), including Gram bacteria such as Pseudomonas aeruginosa and Escherichia coli, as well as Gram bacteria like Staphylococcus aureus and Streptococcus pneumoniae. The action can be ascribed to the unique biological and physicochemical features of AgNPs, which facilitate the disruption of bacterial cell membranes. The UV-visible analysis solution after the introduction of AgNPs indicated that the decrease in the absorbance peak of methyl red was attributed to the existence of silver nano-particles. Metal nano-particles can be synthesized using environmentally friendly processes and hold great potential for combating multi-drug resistant bacteria and degrading Azo dyes. Silver nano-particles (AgNPs) are synthesized with an extract derived from the algae Spirulina platensis, which is a sustainable and eco-friendly alternative.
确保每个人在各个年龄段都能享有健康的生活方式和幸福安康，在增加清洁水和卫生设施的可及性以及减少流行病和疾病传播方面已取得进展。利用微藻合成纳米颗粒（NPs）是一种新的纳米生物技术，因为使用微藻的生物分子（冠层）作为NP生成的封端和还原剂。本研究探讨了一种独特的本土微藻菌株合成银纳米颗粒（AgNPs）的能力，以及其对多药耐药（MDR）细菌的有效性及其在废水中降解偶氮染料（甲基红）的能力。从当地来源获得钝顶螺旋藻提取物以合成银纳米颗粒（AgNPs）。随后，利用几种分析方法对合成的AgNPs进行表征，即紫外可见光谱、扫描电子显微镜（SEM）、X射线衍射和傅里叶变换红外光谱（FTIR分析）。随后，采用纸片扩散法评估其对多药耐药（MDR）细菌的抗菌效果及其在废水中降解偶氮染料（甲基红）的能力。通过生物合成产生的纳米颗粒在紫外可见光谱中在430nm波长处呈现出一个突出的峰值。此外，这些纳米颗粒被确定具有晶体性质，平均尺寸为28.72nm，形状独特呈星形。合成的银纳米颗粒（AgNPs）对一些临床细菌分离株如多药耐药（MDR）的细菌表现出剂量依赖性抗菌作用，包括革兰氏阴性菌如铜绿假单胞菌和大肠杆菌，以及革兰氏阳性菌如金黄色葡萄球菌和肺炎链球菌。这种作用可归因于AgNPs独特的生物学和物理化学特性，这些特性有助于破坏细菌细胞膜。引入AgNPs后的紫外可见分析溶液表明，甲基红吸光度峰值的降低归因于银纳米颗粒的存在。金属纳米颗粒可以通过环境友好的工艺合成，在对抗多药耐药细菌和降解偶氮染料方面具有巨大潜力。银纳米颗粒（AgNPs）是用来自钝顶螺旋藻的提取物合成的，这是一种可持续且环保的替代方法。
