Khan Khushdil, Malik Khafsa, Ahmad Mushtaq, Raja M Naveed Iqbal, Nazish Moona, Kamal Asif, Albasher Gadah, Ahmad Shabir, Naseem Muhammad Tahir
Department of Botany, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, 46000, Pakistan.
Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.
Sci Rep. 2024 Dec 30;14(1):31626. doi: 10.1038/s41598-024-79672-w.
Natural honey is enriched with essential and beneficial nutrients. This study aimed to investigate the melliferous flora microscopic techniques and assess the biochemical properties of honey. Flavonoid and phenolic contents in honey samples were analyzed via colorimetric and Folin-Ciocalteu methods and the alpha-amylase, reducing power, and minerals using Pull's and spectroscopy methods. HPLC determined the Sucrose, fructose, and glucose content in the honey samples. Four different classes of moisture content were used to assist the honey quality, including A + < 17, A = 17-18, B = 18-19, and C > 19. This study identified the Eucalyptus globulus, Trifolium pratense, Neltuma juliflora, Ziziphus mauritiana, Asphodelus tenuifolius, Cynodon dactylon, Saccharum spontaneum, and Vachellia nilotica as the predominant plant species honey samples. The total range of moisture was observed from 16.5 to 21.1% in the samples studied. The honey class within the range of 16.5-17.5% was identified as the optimal class. Four classes of sucrose level were used to evaluate the honey quality, including: (2.50-2.60 = A +); (2.61-2.75 = A); (2.76-3.00 = B); (3.00 + = C). The total variation of sucrose levels in the analyzed ranged from 2.50 to 3.89%. Overall, the findings of these studies implemented various aspects of honey production, quality, and potential health benefits, benefiting consumers, beekeepers, and researchers.
天然蜂蜜富含必需的有益营养成分。本研究旨在调查蜜源植物微观技术并评估蜂蜜的生化特性。通过比色法和福林 - 西奥尔特法分析蜂蜜样品中的黄酮类和酚类含量,使用普尔法和光谱法分析α - 淀粉酶、还原力和矿物质。高效液相色谱法测定蜂蜜样品中的蔗糖、果糖和葡萄糖含量。使用四类不同的水分含量来辅助评估蜂蜜质量,包括A + < 17、A = 17 - 18、B = 18 - 19和C > 19。本研究确定蓝桉、红车轴草、垂枝红千层、毛叶枣、细叶阿斯phodelus tenuifolius、狗牙根、甜根子草和阿拉伯金合欢为蜂蜜样品中的主要植物种类。在所研究的样品中,水分含量的总范围为16.5%至21.1%。16.5 - 17.5%范围内的蜂蜜类别被确定为最佳类别。使用四类蔗糖水平来评估蜂蜜质量,包括:(2.50 - 2.60 = A +);(2.61 - 2.75 = A);(2.76 - 3.00 = B);(3.00 + = C)。分析中蔗糖水平的总变化范围为2.50%至3.89%。总体而言,这些研究结果涉及蜂蜜生产、质量和潜在健康益处的各个方面,对消费者、养蜂人和研究人员都有益处。