Ahmad Shabir, Zafar Muhammad, Ahmad Mushtaq, Yaseen Ghulam, Sultana Shazia
Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Microsc Res Tech. 2019 Jun;82(6):720-730. doi: 10.1002/jemt.23218. Epub 2019 Jan 24.
The present study was carried out to investigate the polliniferous bee flora of Lakki Marwat district, Khyber Pakhtunkhwa, Pakistan. A total of 18 species, belonging to 13 different families were identified for the foraging activities of honeybees, namely, Cenchrus biflorus, Cestrum nocturnum, Citrus limon, Combretum indicum, Datura innoxia, Duranta erecta, Hamelia patens, Helianthus annuus, Ipomoea cairica, Luffa aegyptiaca, Nerium oleander, Ocimum basilicum, Parthenium hysterophorus, Pennisetum typhoides, Prosopis cineraria, Prosopis juliflora, Saccharum spontaneum, and Ziziphus jujuba. The identified melliferous flora was collected for the morpho-palynological investigation. Pollen were acetolyzed, measured, and described qualitatively using light microscopy. The pollen types varied from psilate to echinate and colpi from tricolpate to hexacolpate. Quantitative characteristics including polar diameter, equatorial diameter, polar to equatorial ratio, colpi length, colpi width, spine length, spine width, and exine thickness of the pollen were determined and analyzed statistically using software IBM SPSS Statistics 20. The results of the present study highlighted the significance of pollen morphology of the bee flora of the area which may lead to the identification of the potential and useful botanical sources for beekeepers and to check the honey quality marketed from the study area.
本研究旨在调查巴基斯坦开伯尔-普赫图赫瓦省拉基马瓦特地区的授粉蜜蜂植物区系。共鉴定出18种属于13个不同科的植物，这些植物为蜜蜂的觅食活动提供花粉，即双花蒺藜、夜香树、柠檬、毛叶榄仁、无刺曼陀罗、黄金叶、希美利、向日葵、五爪金龙、埃及丝瓜、夹竹桃、罗勒、银胶菊、珍珠粟、灰叶豆、牧豆树、甜根子草和枣。采集已鉴定的蜜源植物用于形态孢粉学研究。花粉经乙酰解处理后，使用光学显微镜进行测量和定性描述。花粉类型从光滑到具刺不等，萌发孔从三沟到六沟。测定了花粉的极轴直径、赤道轴直径、极轴与赤道轴比值、萌发孔长度、萌发孔宽度、刺长度、刺宽度和外壁厚度等定量特征，并使用IBM SPSS Statistics 20软件进行统计分析。本研究结果突出了该地区蜜蜂植物区系花粉形态的重要性，这可能有助于为养蜂人识别潜在且有用的植物来源，并检查该研究地区销售的蜂蜜质量。
