Wu Derek Griffin, D'Amico Vincent, Trammell Tara Lynn Eckard
Department of Plant and Soil Sciences, University of Delaware, Newark, DE, USA.
Population Biology, Ecology, and Evolution Graduate Program, Emory University, Atlanta, GA, USA.
Sci Rep. 2024 Dec 30;14(1):31735. doi: 10.1038/s41598-024-81838-5.
Urban forest soils are complex environments subjected to various stressors that alter chemical and microbial properties. To understand soil chemistry and bacterial community patterns in urban forest soils with respect to site identity and multiflora rose (Rosa multiflora) invasion, soils were collected from beneath R. multiflora, native spice bush (Lindera benzoin), and uncovered ground in three forests in Newark, Delaware. High-throughput sequencing was used to analyze bacterial communities with corresponding soil chemical properties. Soil chemistry and operational taxonomic unit (OTU) communities were explained by site rather than by shrub cover type. Unlike other invasive plant studies, R. multiflora had minimal effects on either soil chemistry or bacterial communities. Phylum level bacterial communities were more uniform under shrub cover versus no cover, indicative of a generalized plant effect shaping soil microbiomes. Correlations between bacterial phyla and soil chemistry varied, with some phyla positively or negatively correlating with the same property at different sites. Filters for bacterial communities differ across forest scales, where sites and sampling location primarily correlate with OTU communities yet shrub presence mediates phylum level organization. Forest soil studies should consider location-based differences in bacterial communities and their correlations with soil chemistry before generalizing outcomes for whole macrosystems.
城市森林土壤是复杂的环境，受到各种压力源的影响，这些压力源会改变土壤的化学和微生物特性。为了了解城市森林土壤中土壤化学和细菌群落模式与场地特征以及多花蔷薇（Rosa multiflora）入侵之间的关系，研究人员从特拉华州纽瓦克市的三片森林中多花蔷薇、本土的山胡椒（Lindera benzoin）下方以及裸露地面采集了土壤样本。利用高通量测序分析细菌群落及其相应的土壤化学性质。土壤化学和操作分类单元（OTU）群落是由场地而非灌木覆盖类型来解释的。与其他入侵植物研究不同，多花蔷薇对土壤化学或细菌群落的影响极小。与无覆盖相比，灌木覆盖下门水平的细菌群落更为均匀，这表明植物对塑造土壤微生物群落具有普遍影响。细菌门与土壤化学之间的相关性各不相同，一些门类在不同场地与相同性质呈正相关或负相关。不同森林尺度下细菌群落的筛选标准不同，场地和采样位置主要与OTU群落相关，而灌木的存在则介导了门水平的组织。在对整个宏观系统的研究结果进行概括之前，森林土壤研究应考虑细菌群落基于位置的差异及其与土壤化学的相关性。
