Effects of nitrogen deposition on soil nitrogen fractions and enzyme activities in wet meadow of the Qinghai-Tibet Plateau.

Soil nitrogen (N) transformation is an essential portion of the N cycle in wetland ecosystems, governing the retention status of soil N by controlling the effective soil N content. N deposition produced by human activities changes the physical characteristics of soil, affecting N fractions and enzyme activities. To characterize these influences, three different N addition levels (N5, 5 g/m; N10, 10 g/m; N15, 15 g/m) were established using a wet meadow on the Qinghai-Tibet Plateau (QTP) as a control treatment (0 g/m). We investigated the features of soil physical property alterations, N fractions contents, and enzyme activities under N addition conditions throughout the peak plant growth season. Our findings indicated that N addition significantly enhanced soil aeration, porosity, total nitrogen (TN), ammonium nitrogen (NH), nitrate nitrogen (NO) content, and urease activity. At the same time, it decreased soil dissolved organic nitrogen (DON) content and bulk density (BD). Additionally, N addition treatment exerted a significant seasonal impact on soil nitrogen component content. The nitrogen component content within the surface soil (0-10 cm) under four treatments is more sensitive to N addition, whereas the nitrogen component in the deep soil is relatively stable. Principal component analysis demonstrated that soil aeration and porosity were the primary factors affecting soil N fractions and enzyme activities. The findings suggested that lower levels of N addition promoted the transformation process of soil N pools in wet meadows and exacerbated the loss of N in wetland ecosystems. Our findings indicate that sustained increases in N deposition will accelerate soil microbial N cycling, potentially overcoming N limitation in alpine wetland ecosystems and exacerbating the risk of N loss and greenhouse gas emissions from alpine wetland surface soils.