Gong Chunying, Ai Junwen, Liu Yong, He Xingjian, Xue Hong, Jia Chaohua, Chen Zhuohua, Xu Hanfu, Liu Rongpeng, Yang Yong
The Sericultural Research Institute of Hunan Province, Changsha, 410127, Hunan, China.
State Key Laboratory of Resource Insects, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing, 400715, China.
Transgenic Res. 2024 Dec 30;34(1):2. doi: 10.1007/s11248-024-00422-9.
Bombyx mori nuclear polyhedrosis, caused by B. mori nucleopolyhedrovirus (BmNPV), threatens sericulture seriously. To explore strategies for controlling it, the UDP glycosyltransferase gene UGT41A3 (BmUGT41A3) was targeted. UGT is involved in exogenous substances detoxification and endogenous biomass regulation in insects. Early embryos of the BmNPV-sensitive variety 'HYB' were used to obtain the transgenic line HYB-UGT41A3, overexpressing BmUGT41A3 under the IE1 promoter. qPCR results revealed that, compared with the wild-type control 'HYB', BmUGT41A3 was upregulated during the individual developmental stages of HYB-UGT41A3 from silkworm eggs to fifth-instar larvae; peak expression was observed in the third-instar larvae, which presented the most significantly upregulated expression. Individual-tissues qPCR results revealed that BmUGT41A3 expression was highest in the hemocytes of HYB-UGT41A3, followed by the midgut, whereas expression in HYB was very low. Gradient feeding of BmNPV on HYB-UGT41A3 and control 'HYB' larvae on the first day of the second-instar stage. The results revealed that the LC of HYB-UGT41A3 reached 4.040 × 10 particles/mL, which was 20-fold greater than that of HYB. The decrease in the BmNPV load was more significant in HYB-UGT41A3 than in HYB at 48 h after viral inoculation. These results indicate BmUGT41A3 overexpression inhibits BmNPV proliferation and improve resistance to BmNPV in B. mori.
家蚕核型多角体病由家蚕核型多角体病毒（BmNPV）引起，对养蚕业构成严重威胁。为探索防治策略，研究针对了UDP糖基转移酶基因UGT41A3（BmUGT41A3）。UGT参与昆虫对外源物质的解毒以及内生物质的调节。利用对BmNPV敏感的品种“HYB”的早期胚胎获得转基因品系HYB - UGT41A3，该品系在IE1启动子的控制下过表达BmUGT41A3。qPCR结果显示，与野生型对照“HYB”相比，从蚕卵到五龄幼虫阶段，HYB - UGT41A3个体发育阶段中BmUGT41A3均上调；在三龄幼虫中观察到表达峰值，其表达上调最为显著。个体组织qPCR结果显示，BmUGT41A3在HYB - UGT41A3的血细胞中表达最高，其次是中肠，而在HYB中的表达非常低。在二龄期第一天对HYB - UGT41A3和对照“HYB”幼虫进行BmNPV的梯度喂食。结果显示，HYB - UGT41A3的半数致死浓度（LC）达到4.040×10颗粒/毫升，比HYB高20倍。病毒接种后48小时，HYB - UGT41A3中BmNPV载量的下降比HYB更显著。这些结果表明，BmUGT41A3的过表达抑制了BmNPV的增殖，并提高了家蚕对BmNPV的抗性。
