Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China.
Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang, China.
Pest Manag Sci. 2022 Dec;78(12):5302-5312. doi: 10.1002/ps.7152. Epub 2022 Sep 12.
Nucleopolyhedrovirus (NPV), one of the baculoviruses, is a promising biopesticide for pest control. Lepidopteran account for 70% of pests, therefore investigation on highly conserved genes associated with viral infections in the lepidopteran model, the silkworm, will serve as a valuable reference for improving the effectiveness of pest management. BmE74A is a member of the erythroblast transformation-specific (ETS) family of transcription factors in Bombyx mori, which we previously found to be highly conserved and closely associated with BmNPV. This study aimed to elucidate the role of BmE74A in viral infection.
A significantly high expression of BmE74A in eggs indicated its important role in embryonic development, as did relatively high expressions in the hemolymph and midgut. Significant differences in BmE74A expression in different resistant strains after BmNPV infection suggested its involvement as a response to viral infection. Moreover, RNA interference (RNAi) and overexpression experiments confirmed the important role of BmE74A in promoting viral infection. BmNPV infection was significantly suppressed and enhanced by BmE74A knockdown and overexpression, respectively. Besides, BmE74A was found to regulate the expression of BmMdm2 and Bmp53. Furthermore, the binding of ETS, the functional domain of BmE74A, to occlusion-derived virus proteins was confirmed by far-western blotting, and four viral proteins that may interact with ETS proteins were identified by mass spectrometry. Similarly, a homolog of BmE74A in Spodoptera litura was also found to be involved in larval susceptibility to BmNPV.
BmE74A promotes BmNPV proliferation by directly interacting with the virus, which may be related to the suppression of the p53 pathway. © 2022 Society of Chemical Industry.
核型多角体病毒（NPV）是杆状病毒的一种，是一种有前途的害虫生物防治剂。鳞翅目昆虫占害虫的 70%，因此对鳞翅目模式昆虫家蚕中与病毒感染相关的高度保守基因进行研究，将为提高害虫管理的有效性提供有价值的参考。BmE74A 是家蚕的红细胞生成素转化特异性（ETS）转录因子家族的一员，我们之前发现它高度保守，与 BmNPV 密切相关。本研究旨在阐明 BmE74A 在病毒感染中的作用。
BmE74A 在卵中的表达水平显著升高，表明其在胚胎发育中具有重要作用，在血液和中肠中的表达水平也相对较高。BmNPV 感染后不同抗性品系中 BmE74A 表达水平的显著差异表明其作为对病毒感染的反应参与其中。此外，RNA 干扰（RNAi）和过表达实验证实了 BmE74A 在促进病毒感染中的重要作用。BmNPV 感染分别被 BmE74A 的敲低和过表达显著抑制和增强。此外，发现 BmE74A 调节 BmMdm2 和 Bmp53 的表达。此外，通过远Western 印迹证实了 ETS，即 BmE74A 的功能域，与包埋衍生病毒蛋白的结合，通过质谱鉴定出 4 种可能与 ETS 蛋白相互作用的病毒蛋白。同样，在斜纹夜蛾中发现 BmE74A 的同源物也参与幼虫对 BmNPV 的敏感性。
BmE74A 通过直接与病毒相互作用促进 BmNPV 的增殖，这可能与 p53 途径的抑制有关。 © 2022 化学工业协会。
