基质吸附层粘连蛋白途径对神经突生长的引导

Guidance of neurite outgrowth by pathways of substratum-adsorbed laminin.

作者信息

Hammarback J A, Palm S L, Furcht L T, Letourneau P C

出版信息

J Neurosci Res. 1985;13(1-2):213-20. doi: 10.1002/jnr.490130115.

Abstract

Neurite outgrowth is guided by narrow pathways of bioactive laminin. These pathways are created by ultraviolet light irradiation of laminin-coated coverslips masked with electron microscope grids. Patterned outgrowth of neurites is independent of gross mechanical guidance and guidance caused by substrate limitation. Cells on unirradiated laminin are less readily displaced by shear forces than cells on irradiated laminin. This study suggests that ultraviolet light alters the adhesive properties of laminin and that differential cell-substratum adhesion may guide extending neurites on the purified naturally occurring substance, laminin.

摘要

神经突生长由生物活性层粘连蛋白的狭窄路径引导。这些路径是通过用电子显微镜网格遮盖的层粘连蛋白包被盖玻片的紫外线照射产生的。神经突的定向生长独立于总体机械引导和由底物限制引起的引导。与照射过的层粘连蛋白上的细胞相比，未照射的层粘连蛋白上的细胞更不容易被剪切力所移动。这项研究表明，紫外线改变了层粘连蛋白的黏附特性，并且不同的细胞-底物黏附可能引导纯化的天然物质层粘连蛋白上延伸的神经突。