Gundersen R W
Dev Biol. 1987 Jun;121(2):423-31. doi: 10.1016/0012-1606(87)90179-5.
During neurite elongation in the developing peripheral nervous system, the distribution of laminin and fibronectin may provide preferred substrates for neurite elongation. In this study, the response of sensory neurites and growth cones to patterns of laminin or fibronectin applied to a background substrate of Type IV collagen was studied to determine any possible substrate preference. Neurites exhibited elongation restricted to a laminin pattern, but not a fibronectin pattern, indicating that sensory neurites prefer to elongate on laminin compared to Type IV collagen. When polylysine is included in the background substrate, neurite preference for laminin is decreased. Laminin also enhances neurite elongation and defasciculation and stabilizes growth cone protrusions. These results suggest an adhesive as well as a cytoskeletal involvement in the response to laminin, but direct adhesion estimates indicate that laminin decreases overall adhesion, arguing against an adhesive involvement. Regardless of the mechanism involved, the observed neurite preference for laminin is consistent with the hypothesis that spatial and temporal laminin distributions provide preferred pathways for peripheral neurite elongation.
在发育中的周围神经系统的神经突伸长过程中，层粘连蛋白和纤连蛋白的分布可能为神经突伸长提供了优先的底物。在本研究中，研究了感觉神经突和生长锥对应用于IV型胶原背景底物上的层粘连蛋白或纤连蛋白图案的反应，以确定任何可能的底物偏好。神经突表现出伸长仅限于层粘连蛋白图案，而非纤连蛋白图案，这表明与IV型胶原相比，感觉神经突更喜欢在层粘连蛋白上伸长。当背景底物中包含聚赖氨酸时，神经突对层粘连蛋白的偏好会降低。层粘连蛋白还能增强神经突伸长和去束状化，并稳定生长锥突起。这些结果表明在对层粘连蛋白的反应中存在黏附以及细胞骨架的参与，但直接的黏附估计表明层粘连蛋白会降低总体黏附，这与黏附参与的观点相悖。无论涉及何种机制，观察到的神经突对层粘连蛋白的偏好与以下假设一致，即层粘连蛋白的空间和时间分布为周围神经突伸长提供了优先途径。
