Arafa P, Hoque M M, Paul S K, Mazid R, Nila S S, Khan S, Paul A, Hossain T, Nasreen S A, Ahmed S, Haque N
Dr Parvez Arafa, Lecturer, Department of Virology, Mymensingh Medical College (MMC), Mymensingh, Bangladesh; E-mail:
Mymensingh Med J. 2025 Jan;34(1):90-97.
Fluroquinolone resistance among the Enterobacteriaceae including Proteus spp. is a serious public health problem as it is used for treating many clinical infections including UTI and intra-abdominal infections. Now-a-days, plasmid- mediated quinolone resistance (PMQR) has presented with considerable attention that can spread from one bacterium to another. So, the aim of this study was to detect the presence of qnr type (qnr A, B, D and S) and aac(6́)-Ib-cr genes among clinical isolates of ciprofloxacin resistance Proteus species .This cross-sectional study was carried out in the Department of Microbiology, Mymensingh Medical College, Mymensingh, Bangladesh from March 2020 to February 2021. Antimicrobial susceptibility test of 60 Proteus spp. isolates were done by disc diffusion technique. Among them, 48(80.0%) ciprofloxacin resistant isolates were detected during disk-diffusion technique and MIC of ciprofloxacin was performed among ciprofloxacin resistant Proteus isolates using agar dilution method. Amplification with uniplex specific primers of quinolone resistance genes (qnrA, qnrB, qnrD, qnrS and aac(6́)-Ib-cr) were performed out of quinolone resistant Proteus spp. isolates. Out of 48 Proteus species, Quinolone resistance genes were found positive 22(45.83%) for aac(6́)-Ib-cr, 9(18.75%) for qnrD, 6(12.5%) for qnrA and 3(6.25%) for qnrS. None of the strain was positive for qnrB gene. In total, 64.58% ciprofloxacin resistant Proteus isolates were positive for at least one PMQR gene. In this study, presence of high rate (64.5%) of PMQR genes as well as co-existence (30.0%) of the multiple genes among Proteus spp. is highly alarming, because fast dissemination could occur due to the increased prescription of fluoroquinolones and conjugative plasmid mediated horizontal transfer.
包括变形杆菌属在内的肠杆菌科细菌对氟喹诺酮类药物的耐药性是一个严重的公共卫生问题，因为氟喹诺酮类药物被用于治疗包括尿路感染和腹腔内感染在内的许多临床感染。如今，质粒介导的喹诺酮耐药性（PMQR）已受到相当多的关注，它可以从一种细菌传播到另一种细菌。因此，本研究的目的是检测环丙沙星耐药变形杆菌临床分离株中qnr类型（qnr A、B、D和S）和aac(6́)-Ib-cr基因的存在情况。这项横断面研究于2020年3月至2021年2月在孟加拉国迈门辛医学院微生物学系进行。采用纸片扩散法对60株变形杆菌属分离株进行了药敏试验。其中，在纸片扩散试验中检测到48株（80.0%）对环丙沙星耐药的分离株，并使用琼脂稀释法对环丙沙星耐药的变形杆菌分离株进行了环丙沙星的最低抑菌浓度检测。从对喹诺酮耐药的变形杆菌属分离株中，使用单重特异性引物对喹诺酮耐药基因（qnrA、qnrB、qnrD、qnrS和aac(6́)-Ib-cr）进行扩增。在48株变形杆菌中，aac(6́)-Ib-cr喹诺酮耐药基因阳性的有22株（45.83%），qnrD阳性的有9株（18.75%），qnrA阳性的有6株（~12.5%），qnrS阳性的有3株（6.25%）。没有菌株qnrB基因呈阳性。总的来说，64.58%的环丙沙星耐药变形杆菌分离株至少有一个PMQR基因呈阳性。在本研究中，变形杆菌属中PMQR基因的高检出率（64.5%）以及多个基因的共存（30.0%）令人高度警惕，因为由于氟喹诺酮类药物处方的增加和接合质粒介导的水平转移，可能会迅速传播。
