Hagele Anthony M, Krieger Joesi M, Gaige Connor J, Holley Kevin F, Gross Kristen N, Iannotti Joshua M, Allen Leah E, Sutton Paige J, Orr Logan S, Mumford Petey W, Purpura Martin, Jager Ralf, Kerksick Chad M
Exercise and Performance Nutrition Laboratory, Kinesiology Department, College of Science, Technology and Health, Lindenwood University, St. Charles, Missouri, United States of America.
Increnovo, LLC, Whitefish Bay, Wisconsin, United States of America.
PLoS One. 2024 Dec 31;19(12):e0312997. doi: 10.1371/journal.pone.0312997. eCollection 2024.
The essential amino acid leucine (LEU) plays a crucial role in promoting resistance-training adaptations. Dileucine (DILEU), a LEU-LEU dipeptide, increases MPS rates, however its impact on resistance training outcomes remains unexplored. This study assessed the effects of DILEU supplementation on resistance training adaptations.
Using a randomized, double-blind, placebo-controlled approach, 34 resistance-trained males (age: 28.3 ± 5.9 years) consumed 2 grams of either DILEU monohydrate (RAMPS™, Ingenious Ingredients, L.P.), LEU, or placebo (PLA) while following a 4-day per week resistance training program for 10 weeks. Changes in body composition, 1-repetition maximum (1RM) and repetitions to failure (RTF) for leg press (LP) and bench press (BP), anaerobic capacity, countermovement jump (CMJ), and maximal voluntary contraction (MVC) were assessed after 0 and 10 weeks.
Significant main effects for time (p < 0.001) were realized for LP and BP 1RM and RTF. A significant group × time interaction was identified for changes in LP 1RM (p = 0.02) and LP RTF (p = 0.03). Greater increases in LP 1RM were observed in DILEU compared to PLA (p = 0.02; 95% CI: 5.8, 73.2 kg), and greater increases in LP RTF in DILEU compared to LEU (p = 0.04; 95% CI: 0.58, 20.3 reps). No significant differences were found in other measures.
DILEU supplementation at 2 grams daily enhanced lower body strength and muscular endurance in resistance-trained males more effectively than LEU or PLA. These findings suggest DILEU as a potentially effective supplement for improving adaptations to resistance training. NCT06121869 retrospectively registered.
必需氨基酸亮氨酸（LEU）在促进抗阻训练适应性方面起着关键作用。双亮氨酸（DILEU），一种亮氨酸 - 亮氨酸二肽，可提高肌肉蛋白质合成（MPS）速率，然而其对抗阻训练效果的影响仍未得到探索。本研究评估了补充DILEU对抗阻训练适应性的影响。
采用随机、双盲、安慰剂对照方法，34名经过抗阻训练的男性（年龄：28.3±5.9岁）在每周进行4天抗阻训练计划，持续10周的同时，服用2克一水合DILEU（RAMPS™，Ingenious Ingredients，L.P.）、亮氨酸或安慰剂（PLA）。在0周和10周后评估身体成分、腿举（LP）和卧推（BP）的1次重复最大值（1RM）和重复至力竭次数（RTF）、无氧能力、纵跳（CMJ）和最大自主收缩（MVC）的变化。
LP和BP的1RM及RTF在时间上有显著的主效应（p<0.001）。在LP的1RM变化（p = 0.02）和LP的RTF变化（p = 0.03）方面发现了显著的组×时间交互作用。与PLA相比，DILEU组的LP的1RM增加幅度更大（p = 0.02；95%CI：5.8，73.2千克），与亮氨酸相比，DILEU组的LP的RTF增加幅度更大（p = 0.04；95%CI：0.58，20.3次）。在其他测量指标上未发现显著差异。
每天补充2克DILEU比亮氨酸或安慰剂更有效地增强了经过抗阻训练男性的下肢力量和肌肉耐力。这些发现表明DILEU是一种潜在有效的补充剂，可改善对抗阻训练的适应性。NCT06121869回顾性注册。
