Chen Shixi, Li Ning, Safiul Azam Fardous Mohammad, Ao Li, Li Na, Wang Jianlan, Zou Yuanchao, Li Rui, Prodhan Zakaria Hossain
College of Life Sciences, Neijiang Normal University, Neijiang, China.
Conservation and Utilization of Fishes resources in the Upper Reaches of the Yangtze River, Key Laboratory of Sichuan Province, Neijiang, China.
PLoS One. 2024 Dec 31;19(12):e0315996. doi: 10.1371/journal.pone.0315996. eCollection 2024.
The albino northern snakehead (Channa argus) is an aquaculture species characterized by heritable albino body color, in contrast to the typical coloration. Additionally, there are gray- and golden-finned individuals, which exhibit distinct coloration in their caudal fins. We performed RNA-seq to profile the transcriptome of caudal fin tissues in albino gray-finned and golden-finned C. argus, contrasting these with normal morphs to elucidate the differences between the two groups. A total of 137,130 unigenes were identified in this study. Gene Ontology (GO) analysis showed that the identified DEGs were significantly enriched in cellular components related to cytoplasm. So far, 379 common DEGs have been identified in all three groups. Notably, we observed more DEGs in golden-finned individuals compared to gray-finned individuals. We also revealed that golden-finned individuals were enriched in collagen-related pathways compared with normal individuals. The enriched DEGs of collagen components include collagen I of COL1A1 and COL1A2, collagen II of COL2A1, collagen V of COL5A1 and COL5A2, collagen VI of COL6A1 and COL6A3, collagen IX of COL9A3, collagen X of COL10A1, collagen XI of COL11A2, collagen XII of COL12A1, collagen XVI of COL16A1, collagen XVIII of COL18A1 and decorin (DCN), all of which play a role in modulating the collagen matrix. In golden-finned albino fish, collagen-related genes were downregulated, suggesting that despite the abundance of collagen types in their caudal fin cells, gene expression was slightly limited. This work provides valuable genetic insights into collagen variation in albino C. argus, lays the foundation for research on collagen genes and is crucial for the development and utilization of fish-derived collagen as a biomaterial for tissue engineering and biomedical applications.
白化北方黑鱼（乌鳢）是一种水产养殖品种，其特征是具有可遗传的白化体色，与典型体色形成对比。此外，还有灰鳍和金鳍个体，它们的尾鳍呈现出独特的颜色。我们进行了RNA测序，以分析白化灰鳍和金鳍乌鳢尾鳍组织的转录组，并将其与正常形态进行对比，以阐明两组之间的差异。本研究共鉴定出137,130个单基因。基因本体论（GO）分析表明，鉴定出的差异表达基因在与细胞质相关的细胞成分中显著富集。到目前为止，在所有三组中已鉴定出379个常见的差异表达基因。值得注意的是，与灰鳍个体相比，我们在金鳍个体中观察到更多的差异表达基因。我们还发现，与正常个体相比，金鳍个体在与胶原蛋白相关的途径中富集。胶原蛋白成分的富集差异表达基因包括COL1A1和COL1A2的I型胶原蛋白、COL2A1的II型胶原蛋白、COL5A1和COL5A2的V型胶原蛋白、COL6A1和COL6A3的VI型胶原蛋白、COL9A3的IX型胶原蛋白、COL10A1的X型胶原蛋白、COL11A2的XI型胶原蛋白、COL12A1的XII型胶原蛋白、COL16A1的XVI型胶原蛋白、COL18A1的XVIII型胶原蛋白和核心蛋白聚糖（DCN），所有这些在调节胶原蛋白基质中发挥作用。在金鳍白化鱼中，与胶原蛋白相关的基因下调，这表明尽管其尾鳍细胞中胶原蛋白类型丰富，但基因表达略有受限。这项工作为白化乌鳢胶原蛋白变异提供了有价值的遗传见解，为胶原蛋白基因的研究奠定了基础，对于将鱼源胶原蛋白开发和利用为组织工程和生物医学应用的生物材料至关重要。
