Wang Chong, Shu Qingsong, Zeng Nanyang, Xie Shaolin, Zou Jixing, Tang Huijuan, Zhou Aiguo
College of Marine Sciences, South China Agricultural University, Guangzhou 510642, Guangdong, China.
College of Marine Sciences, South China Agricultural University, Guangzhou 510642, Guangdong, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2024 Dec;52:101321. doi: 10.1016/j.cbd.2024.101321. Epub 2024 Sep 7.
To compare and analyze the differences in immunological response between the two color morphs of Channa argus, a fish cohort was divided into four groups: black C argus + PBS (B-PBS), black C argus + Aeromonas hydrophila (B-Ah), white C. argus + PBS (W-PBS), and white C. argus + A hydrophila (W-Ah). The B-PBS and W-PBS groups received 100 μL PBS, while the B-Ah and W-Ah groups received 3.6 × 10 CFU/mL A. hydrophila in the same volume. The death rate in each group was noted, changes in plasma biochemical indicators and the expression of liver immune-related genes were examined, and transcriptome techniques were used to compare the differences between the two colors of C. argus following stress. No mortality occurred in the B-PBS and W-PBS groups. Mortality in the W-Ah and B-Ah groups showed an upward and then downward trend after A. hydrophila injection. The highest mortality occurred within 24 h and was higher in the W-Ah group than in the B-Ah group. MDA levels in the B-Ah and W-Ah groups increased and then decreased, while SOD and T-AOC showed the reverse tendency. The W-Ah and W-PBS groups differed significantly in MDA at 3, 12, and 24 h, SOD from 6 to 96 h, and T-AOC between 6 and 48 h. Plasma MDA and T-AOC levels at 12 h and SOD levels at 24 and 48 h differed significantly between the B-PBS and B-Ah groups. In both the W-Ah and B-Ah groups, the expression levels of IL-1β and IL-8 in the liver showed a temporal pattern with an initial increase followed by a decrease, reaching peak levels after 24 h, while IL-10 showed the reverse pattern. Transcriptome analysis of the liver revealed significant differences between the two C. argus colors. Differential genes in black C. argus were mainly enriched in steroid biosynthesis, glycolysis/gluconeogenesis, and glutathione and propanoate metabolism pathways 24 h after infection. In contrast, differential genes in white C. argus were mainly enriched in pathways such as oxidative phosphorylation, pancreatic secretion, and protein digestion and absorption 24 h after infection. After A. hydrophila infection, white C. argus had higher mortality, more severe oxidative stress and inflammatory responses, and lower antioxidant capacity than black C. argus.
为了比较和分析乌鳢两种体色形态之间免疫反应的差异，将一组鱼分为四组：黑色乌鳢+磷酸盐缓冲液（B-PBS）、黑色乌鳢+嗜水气单胞菌（B-Ah）、白色乌鳢+磷酸盐缓冲液（W-PBS）和白色乌鳢+嗜水气单胞菌（W-Ah）。B-PBS组和W-PBS组注射100μL磷酸盐缓冲液，而B-Ah组和W-Ah组注射相同体积的浓度为3.6×10CFU/mL的嗜水气单胞菌。记录每组的死亡率，检测血浆生化指标变化及肝脏免疫相关基因的表达，并采用转录组技术比较应激后两种体色乌鳢之间的差异。B-PBS组和W-PBS组均未出现死亡。W-Ah组和B-Ah组在注射嗜水气单胞菌后死亡率呈先上升后下降趋势。最高死亡率出现在24小时内，且W-Ah组高于B-Ah组。B-Ah组和W-Ah组的丙二醛（MDA）水平先升高后降低，而超氧化物歧化酶（SOD）和总抗氧化能力（T-AOC）则呈相反趋势。W-Ah组和W-PBS组在3、12和24小时时MDA水平、6至96小时时SOD水平以及6至48小时时T-AOC水平存在显著差异。B-PBS组和B-Ah组在12小时时血浆MDA和T-AOC水平以及24和48小时时SOD水平存在显著差异。在W-Ah组和B-Ah组中，肝脏中白细胞介素-1β（IL-1β）和白细胞介素-8（IL-8）的表达水平呈现先升高后降低的时间模式，在24小时后达到峰值水平，而白细胞介素-10（IL-10）则呈现相反模式。肝脏转录组分析显示两种体色乌鳢之间存在显著差异。感染24小时后，黑色乌鳢中的差异基因主要富集在类固醇生物合成、糖酵解/糖异生以及谷胱甘肽和丙酸代谢途径中。相比之下，感染24小时后，白色乌鳢中的差异基因主要富集在氧化磷酸化、胰腺分泌以及蛋白质消化和吸收等途径中。感染嗜水气单胞菌后，白色乌鳢比黑色乌鳢具有更高的死亡率、更严重的氧化应激和炎症反应以及更低的抗氧化能力。
