Microcalorimetry studies of energy changes during the growth of Klebsiella aerogenes in simple salts/glucose media. 1 Establishment of standard conditions.

The shape of the thermogram and the total heat output of aerobically growing cultures of Klebsiella aerogenes depend on the nature and state of the inoculum, the composition of the growth medium, aeration in the fermentor and in the calorimeter, and the pump rate of the culture through the microcalorimeter cell. Standard conditions of growth and heat measurement have been established which give reproducible thermograms and total heat output. Experimental results using different glucose concentrations are reported and the enthalpy changes for the consumption of glucose and the production of cells have been calculated.