Yang Shuiyue, Guo Jia, Chen Danyang, Sun Zepeng, Pu Li, Sun Guoying, Yang Min, Peng Yinghua
Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin 130112, China.
School of Chemistry and Life Science, Changchun University of Technology, Changchun, Jilin 130012, China.
ACS Appl Bio Mater. 2025 Jan 20;8(1):814-824. doi: 10.1021/acsabm.4c01644. Epub 2024 Dec 31.
Ginsenosides possess potential protective effects against cisplatin (CDDP)-induced toxicity, but the limited bioavailability of ginsenosides hampered their therapeutic application. Ginseng exosomes (G-Exo), which are active ingredients in ginseng, exhibit excellent biocompatibility and low immunogenicity. Here, G-Exo were isolated from ginseng roots through a combination of ultracentrifugation and sucrose gradient centrifugation techniques. Subsequently, the potential protective effect of G-Exo on CDDP induced cardiotoxicity, and its underlying mechanisms were explored. The findings demonstrated that G-Exo effectively mitigated CDDP-induced oxidative stress and apoptosis . Moreover, experiments revealed that G-Exo significantly inhibited the increases in serum cardiac troponin T (cTnT), creatine kinase (CK), and lactate dehydrogenase (LDH) levels in mice induced by CDDP. Histological assessment and tissue staining further corroborated that G-Exo alleviated the cardiac tissue damage and apoptosis caused by CDDP. Mechanistically, G-Exo were found to alleviate CDDP-induced apoptosis through blocking the MAPK signaling. Collectively, these results suggest that G-Exo hold the potential to mitigate cisplatin-induced cardiac injury by regulating the MAPK pathway, thereby highlighting the therapeutic potential of G-Exo as a protective agent against CDDP-induced cardiotoxicity.
人参皂苷对顺铂（CDDP）诱导的毒性具有潜在的保护作用，但人参皂苷有限的生物利用度阻碍了它们的治疗应用。人参外泌体（G-Exo）作为人参中的活性成分，具有优异的生物相容性和低免疫原性。在此，通过超速离心和蔗糖梯度离心技术相结合的方法从人参根中分离出G-Exo。随后，探讨了G-Exo对CDDP诱导的心脏毒性的潜在保护作用及其潜在机制。研究结果表明，G-Exo有效减轻了CDDP诱导的氧化应激和细胞凋亡。此外，实验表明G-Exo显著抑制了CDDP诱导的小鼠血清心肌肌钙蛋白T（cTnT）、肌酸激酶（CK）和乳酸脱氢酶（LDH）水平的升高。组织学评估和组织染色进一步证实，G-Exo减轻了CDDP引起的心脏组织损伤和细胞凋亡。从机制上讲，发现G-Exo通过阻断MAPK信号传导减轻CDDP诱导的细胞凋亡。总的来说，这些结果表明，G-Exo有潜力通过调节MAPK途径减轻顺铂诱导的心脏损伤，从而突出了G-Exo作为一种抗CDDP诱导心脏毒性保护剂的治疗潜力。
