Bhushan Sakchi, Singh Sandhya, Maiti Tushar Kanti, Das Ankita, Barui Ananya, Chaudhari Leena R, Joshi Meghnad G, Dutt Dharm
Department of Paper Technology, IIT Roorkee, Saharanpur 247001, India.
Department of Polymer and Process Engineering, IIT Roorkee, Saharanpur 247001, India.
Biomed Mater. 2025 Jan 16;20(2). doi: 10.1088/1748-605X/ada477.
The advancement in the arena of bone tissue engineering persuades us to develop novel nanocomposite scaffolds in order to improve antibacterial, osteogenic, and angiogenic properties that show resemblance to natural bone extracellular matrix. Here, we focused on the development of novel zinc-doped hydroxyapatite (ZnHAP) nanoparticles (1, 2 and 3 wt%; size: 50-60 nm) incorporated chitosan-gelatin (CG) nanocomposite scaffold, with an interconnected porous structure. The addition of ZnHAP nanoparticles decreases the pore size (∼30 µm) of the CG scaffolds. It was observed that with the increase in the concentration of ZnHAP nanoparticles (3 wt%) in CG scaffolds, the swelling ratio (1760% ± 2.0%), porosity (71% ± 0.98%) and degradation rate (35%) decreased, whereas mechanical property (1 MPa) increased, which was better as compared to control (CG) samples. Similarly, the high deposition of apatite crystals especially CG-ZnHAPnanocomposite scaffold revealed the excellent osteoconductive potential among all other scaffolds. MC3T3-E1 osteoblastic cells seeded with CG-ZnHAP nanocomposite scaffolds depicted better cell adhesion, proliferation and differentiation to osteogenic lineages. Finally, the chorioallantoic membrane (CAM) assay revealed better angiogenesis of ZnHAP nanoparticles (3 wt%) loaded CG scaffolds supporting vascularization after 7th day incubation in the CAM area. Overall, the results showed that the CG-ZnHAPnanocomposite scaffold could be a potential candidate for bone defect repair.
骨组织工程领域的进展促使我们开发新型纳米复合支架，以改善其抗菌、成骨和血管生成特性，使其与天然骨细胞外基质相似。在此，我们专注于开发新型掺锌羟基磷灰石（ZnHAP）纳米颗粒（1%、2%和3%重量比；尺寸：50 - 60纳米）与壳聚糖 - 明胶（CG）复合的纳米复合支架，其具有相互连接的多孔结构。ZnHAP纳米颗粒的添加减小了CG支架的孔径（约30微米）。观察到随着CG支架中ZnHAP纳米颗粒浓度（3%重量比）的增加，溶胀率（1760% ± 2.0%）、孔隙率（71% ± 0.98%）和降解率（35%）降低，而机械性能（1兆帕）提高，与对照（CG）样品相比更好。同样，磷灰石晶体的高沉积，尤其是CG - ZnHAP纳米复合支架在所有其他支架中显示出优异的骨传导潜力。接种在CG - ZnHAP纳米复合支架上的MC3T3 - E1成骨细胞表现出更好的细胞黏附、增殖以及向成骨谱系的分化。最后，绒毛尿囊膜（CAM）试验表明，在CAM区域培养7天后，负载3%重量比ZnHAP纳米颗粒的CG支架具有更好的血管生成能力，支持血管化。总体而言，结果表明CG - ZnHAP纳米复合支架可能是骨缺损修复的潜在候选材料。
