Chen Qing-Bin, Sun Xiao-Yu, Zheng Meng-Yao, Liu Ya-Nan, Zhang Jin-Xiu, Zhou Qing-Feng, Pei Dong-Li, Liu Dong-Mei, Chen Ya-Wei, Gao Hang, Xing Xiao-Long, Jiang Hao, Wang Xue-Ling, Yuan Li, Wang Wen-Jing
Henan Provincial Engineering Research Center for Development and Application of Characteristic Microorganism Resources, Dried Chili Industry Technology Research Center, Department of Biology and Food Science, Shangqiu Normal University, Shangqiu, Henan 476000, China.
Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, Guangdong 518120, China.
Int J Biol Macromol. 2025 Mar;292:139315. doi: 10.1016/j.ijbiomac.2024.139315. Epub 2024 Dec 29.
Plants frequently encounter phosphate (Pi) starvation due to its scarce availability in soil, necessitating an adaptive phosphate starvation response (PSR). This study explores this adaptation in pepper (Capsicum annuum L.) under low-Pi stress, focusing on the PHOSPHATE STARVATION RESPONSE (PHR) gene family. We observably halted shoot growth but promoted root elongation in pepper seedlings under low-Pi conditions, significantly impacting regulatory networks. Our research identified 13 PHR transcription factors in pepper, particularly noting that CaPHR3 rapidly up-regulates in response to low-Pi stress. Overexpressing CaPHR3 in Arabidopsis thaliana enhanced Pi starvation tolerance by modulating PSR-related genes and mitigated hypersensitivity in the Atphr1phl1 double mutant. Furthermore, CaPHR3 binds to the P1BS motif in the pepper PHOSPHATE TRANSPORTER 1;4 (PHT1;4) promoter to boost its expression under Pi deficiency. This activation increased Pi uptake and starvation tolerance when overexpressed. Overall, we pinpointed key players in the PSR mechanism through the CaPHR3-CaPHT1;4 pathway, contributing significantly to our understanding of Pi homeostasis and adaptive strategies in pepper under Pi-deficient conditions.
由于土壤中磷（Pi）的有效性稀缺，植物经常遭遇磷饥饿，因此需要一种适应性的磷饥饿响应（PSR）。本研究探讨了低磷胁迫下辣椒（Capsicum annuum L.）的这种适应性，重点关注磷饥饿响应（PHR）基因家族。我们观察到，在低磷条件下，辣椒幼苗的地上部生长停止，但根系伸长得到促进，这对调控网络产生了显著影响。我们的研究在辣椒中鉴定出13个PHR转录因子，特别指出CaPHR3在低磷胁迫下迅速上调。在拟南芥中过表达CaPHR3通过调节PSR相关基因增强了对磷饥饿的耐受性，并减轻了Atphr1phl1双突变体中的超敏反应。此外，CaPHR3与辣椒磷酸盐转运体1;4（PHT1;4）启动子中的P1BS基序结合，以在磷缺乏时促进其表达。过表达时，这种激活增加了磷的吸收和对饥饿的耐受性。总体而言，我们通过CaPHR3-CaPHT1;4途径确定了PSR机制中的关键参与者，这对我们理解缺磷条件下辣椒的磷稳态和适应性策略有重大贡献。
