Ni Ying, Xie Kailing, Shi Minghui, Shan Hanchen, Wu Wenxiang, Wang Weiwei, Cheng Beijiu, Li Xiaoyu
Key Laboratory of Crop Stress Resistance and High Quality Biology of Anhui Province, Anhui Agricultural University, Hefei 230036, China.
College of Biology and Food Engineering, Suzhou University, Suzhou 234000, China.
Plants (Basel). 2025 May 12;14(10):1443. doi: 10.3390/plants14101443.
Phosphorus, as an essential nutrient, plays an important role in plant growth and development. Although Phosphate Starvation Response 1 (PHR1) or PHR1-like have been recognized as central regulators of phosphorus (Pi) homeostasis in several plants, they have not been systematically studied in Cucurbitaceae. In this study, 11, 10, 8, 12, 12, and 22 genes were identified in cucumber, melon, bottle gourd, watermelon, wax gourd, and pumpkin, respectively, by genome-wide analysis. Phylogenetic analysis showed that the Cucurbitaceae genes were divided into seven distinct subfamilies. These genes were further phylogenetically analyzed for their chromosomal localization, gene structure, protein structure, and synteny. Genomic homology analysis showed that many genes existed in the corresponding homology blocks of six Cucurbitaceae species. qRT-PCR analysis showed that the genes exhibited differential expression under different concentrations of phosphate treatment. Transcriptional self-activation assays showed that CmoPHR2, CmoPHR9, CmoPHR16, and CmoPHR17 proteins had transcriptional self-activating activity. The results of this study provide a basis for the further cloning and functional validation of genes related to the phosphate regulatory network in pumpkin.
磷作为一种必需营养素，在植物生长发育中发挥着重要作用。尽管磷饥饿响应1（PHR1）或类PHR1已被认为是几种植物中磷（Pi）稳态的核心调节因子，但它们在葫芦科中尚未得到系统研究。在本研究中，通过全基因组分析，分别在黄瓜、甜瓜、瓠瓜、西瓜、冬瓜和南瓜中鉴定出11个、10个、8个、12个、12个和22个基因。系统发育分析表明，葫芦科基因分为七个不同的亚家族。对这些基因的染色体定位、基因结构、蛋白质结构和共线性进行了进一步的系统发育分析。基因组同源性分析表明，六个葫芦科物种的相应同源区域中存在许多基因。qRT-PCR分析表明，这些基因在不同浓度的磷酸盐处理下表现出差异表达。转录自激活分析表明，CmoPHR2、CmoPHR9、CmoPHR16和CmoPHR17蛋白具有转录自激活活性。本研究结果为进一步克隆和功能验证南瓜中与磷调节网络相关的基因提供了依据。
