Duan Qiuting, Zhang Yijing, Li Zizhuo, Xu Yini, Xu Ya, Yang Dezhi, Yang Yaling
Department of Clinical Laboratory, The First People's Hospital of Yunnan Province/The Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan, China.
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, Yunnan, China.
Int J Biol Macromol. 2025 Mar;292:139352. doi: 10.1016/j.ijbiomac.2024.139352. Epub 2024 Dec 29.
Human dental caries is an intractable biofilm-associated disease caused by the symbiotic cariogenic bacteria, but how to target effectively eliminate cariogenic bacterial and their biofilms without affecting normal bacteria still remains great challenges. To address this issue, we reported Cu,Fe-doped chitosan-based nanozyme (i.e. CS@Cu,Fe) that exhibits well peroxidase-like activity at acidic environment of caries, and kill S. mutans and S. sanguinis without impacting the normal S. oralis. The synergistic interaction between Cu and Fe could effectively enhance the efficiency of electron transfer, promoting the production of hydroxyl radicals (·OH) and superoxide radical (·O) to selectively destroy the biofilm of S. mutans. Compared to curcumin and hexadecyl trimethyl ammonium bromide (CTAB) (control), the chitosan on the surface of CS@Cu,Fe not only showed the synergistic antibacterial activity, but also enabled the selectively eradication of S. mutans biofilm without affecting S. sanguinis and S. oralis biofilms. Furthermore, CS@Cu,Fe also exhibited excellent selective anti-symbiotic caries bacteria and targeted anti-biofilm properties to hybrid biofilm model of these co-existing bacteria under the same oral environment. Therefore, the CS@Cu,Fe nanozyme not only has potential for the treatment of dental biofilms, but also can offer new insights for the design of highly selective antibacterial and antibiofilm nanozyme.
人类龋齿是一种由共生致龋菌引起的难治性生物膜相关疾病，但如何在不影响正常细菌的情况下有效靶向消除致龋菌及其生物膜仍然是巨大的挑战。为了解决这个问题，我们报道了基于铜铁掺杂壳聚糖的纳米酶（即CS@Cu,Fe），它在龋齿的酸性环境中表现出良好的过氧化物酶样活性，能够杀死变形链球菌和血链球菌，而不影响正常的口腔链球菌。铜和铁之间的协同相互作用可以有效提高电子转移效率，促进羟基自由基（·OH）和超氧自由基（·O）的产生，从而选择性地破坏变形链球菌的生物膜。与姜黄素和十六烷基三甲基溴化铵（CTAB）（对照）相比，CS@Cu,Fe表面的壳聚糖不仅表现出协同抗菌活性，还能在不影响血链球菌和口腔链球菌生物膜的情况下选择性地根除变形链球菌生物膜。此外，在相同口腔环境下，CS@Cu,Fe对这些共存细菌的混合生物膜模型也表现出优异的选择性抗共生致龋菌和靶向抗生物膜特性。因此，CS@Cu,Fe纳米酶不仅具有治疗口腔生物膜的潜力，还能为设计高选择性抗菌和抗生物膜纳米酶提供新的思路。
