Jung Ji-Eun, Cai Jian-Na, Cho Sung-Dae, Song Kwang-Yeob, Jeon Jae-Gyu
a Department of Preventive Dentistry, School of Dentistry, Institute of Oral Bioscience and BK 21 Plus Program , Chonbuk National University , Jeonju , Republic of Korea.
b Department of Oral Pathology, School of Dentistry, Institute of Oral Bioscience and BK 21 Plus Program , Chonbuk National University , Jeonju , Republic of Korea.
Biofouling. 2016 Oct;32(9):1079-87. doi: 10.1080/08927014.2016.1230607.
Despite the widespread use of fluoride for the prevention of dental caries, few studies have demonstrated the effects of fluoride on the bacterial composition of dental biofilms. This study investigated whether fluoride affects the proportion of Streptococcus mutans and S. oralis in mono- and dual-species biofilm models, via microbiological, biochemical, and confocal fluorescence microscope studies. Fluoride did not affect the bacterial count and bio-volume of S. mutans and S. oralis in mono-species biofilms, except for the 24-h-old S. mutans biofilms. However, fluoride reduced the proportion and bio-volume of S. mutans but did not decrease those of S. oralis during both S. oralis and S. mutans dual-species biofilm formation, which may be related to the decrease in extracellular polysaccharide formation by fluoride. These results suggest that fluoride may prevent the shift in the microbial proportion to cariogenic bacteria in dental biofilms, subsequently inhibiting the cariogenic bacteria dominant biofilm formation.
尽管氟化物广泛用于预防龋齿，但很少有研究证明氟化物对牙菌斑细菌组成的影响。本研究通过微生物学、生物化学和共聚焦荧光显微镜研究，调查了氟化物是否会影响单菌种和双菌种生物膜模型中变形链球菌和口腔链球菌的比例。除了24小时龄的变形链球菌生物膜外，氟化物不影响单菌种生物膜中变形链球菌和口腔链球菌的细菌数量和生物量。然而，在口腔链球菌和变形链球菌双菌种生物膜形成过程中，氟化物降低了变形链球菌的比例和生物量，但没有降低口腔链球菌的比例和生物量，这可能与氟化物减少细胞外多糖形成有关。这些结果表明，氟化物可能会阻止牙菌斑中微生物比例向致龋菌的转变，从而抑制以致龋菌为主的生物膜形成。
