Lee Yurimi, Kim Hyun-Soo
Department of Pathology and Translational Genomics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
Department of Pathology and Translational Genomics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea
In Vivo. 2025 Jan-Feb;39(1):367-374. doi: 10.21873/invivo.13837.
BACKGROUND/AIM: Dysregulation of claudin 6 (CLDN6) expression has been widely documented in various malignancies. CLDN6 is aberrantly expressed in many types of human carcinomas; however, its clinical significance in endometrial carcinoma has seldom been investigated. This study aimed to examine the immunohistochemical expression status of CLDN6 in low-grade, early-stage endometrioid endometrial carcinoma (LGES-EEC) and to assess its clinicopathological significance.
We performed immunostaining for CLDN6 in 118 tissue samples from LGES-EECs. Protein expression levels were interpreted using a semi-quantitative histoscore method. All statistical analyses were performed.
CLDN6 was primarily localized along the membranes of the tumor cells. We considered histoscore ≥10 (the staining proportion ≥5% and intensity ≥2) as positive immunoreactivity for CLDN6. Twenty-six of the 118 patients (22.0%) showed CLDN6 positivity. Positive CLDN6 expression was significantly associated with deeper myometrial invasion (p=0.001), higher initial stage (p=0.015), and substantial lymphovascular space invasion (p=0.018).
Aberrant CLDN6 expression is involved in tumor progression in LGES-EECs. In addition, targeting CLDN6 may offer clinical utility in patients with endometrial carcinoma.
背景/目的：紧密连接蛋白6（CLDN6）表达失调在多种恶性肿瘤中已有广泛报道。CLDN6在多种人类癌症中异常表达；然而，其在子宫内膜癌中的临床意义鲜有研究。本研究旨在检测CLDN6在低级别、早期子宫内膜样腺癌（LGES-EEC）中的免疫组化表达情况，并评估其临床病理意义。
我们对118例LGES-EEC组织样本进行了CLDN6免疫染色。采用半定量组织评分法解读蛋白表达水平。进行了所有统计分析。
CLDN6主要定位于肿瘤细胞膜。我们将组织评分≥10（染色比例≥5%且强度≥2）视为CLDN6阳性免疫反应。118例患者中有26例（22.0%）显示CLDN6阳性。CLDN6阳性表达与更深的肌层浸润（p=0.001）、更高的初始分期（p=0.015）和大量淋巴管间隙浸润（p=0.018）显著相关。
CLDN6异常表达参与LGES-EEC的肿瘤进展。此外，靶向CLDN6可能对子宫内膜癌患者具有临床应用价值。
