Kralova Marketa, Jirasko Michal, Dedeckova Eva, Hatakova Hedvika, Broz Pavel, Simanek Vaclav, Slouka David, Pecen Ladislav, Kucera Radek
Department of Pharmacology and Toxicology, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic.
Department of Immunochemistry Diagnostics, University Hospital and Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic.
In Vivo. 2025 Jan-Feb;39(1):452-458. doi: 10.21873/invivo.13848.
BACKGROUND/AIM: Low levels of vitamin D are a widespread global issue. This study aimed to determine the optimal vitamin D3 supplementation dose for healthy young adults by comparing the effectiveness of gradually increasing cholecalciferol doses over two years.
Thirty-five volunteers participated in a two-season pilot study conducted from October to April to avoid sunlight-induced vitamin D3 synthesis. The participants used oil-based drops of cholecalciferol, increasing their dose from 1,000 to 2,000, 4,000, and then 8,000 IU daily for 60 days with a 30-day break.
Supplementing with 1,000 IU/day raised vitamin D levels to the recommended range (above 75 nmol/l), but levels dropped below this range after a 30-day break. A dose of 2,000 IU/day maintained vitamin D levels within the recommended range, even after the break. Increasing the dose to 4,000 IU/day produced a rapid rise, though levels dropped more significantly after stopping supplementation. With 8,000 IU/day, both the rise and subsequent decline in vitamin D levels were more pronounced.
Effective vitamin D supplementation in healthy young adults can be achieved with a daily dose of 2,000 IU during winter. However, 4,000 IU/day was more effective for maintaining levels above 100 nmol/l, supporting broader health benefits. Regular monitoring of [25(OH)D], calcium, and phosphorus levels is essential.
背景/目的：维生素D水平低是一个全球性普遍问题。本研究旨在通过比较两年内逐渐增加胆钙化醇剂量的效果，确定健康年轻成年人维生素D3的最佳补充剂量。
35名志愿者参与了一项从10月至4月进行的两季试点研究，以避免阳光诱导的维生素D3合成。参与者使用油基胆钙化醇滴剂，剂量从每日1000国际单位增加到2000、4000，然后8000国际单位，持续60天，中间休息30天。
每日补充1000国际单位可使维生素D水平升至推荐范围（高于75纳摩尔/升），但休息30天后水平降至该范围以下。每日2000国际单位的剂量即使在休息后也能将维生素D水平维持在推荐范围内。将剂量增加到每日4000国际单位会使维生素D水平迅速上升，不过停止补充后下降更显著。每日8000国际单位时，维生素D水平的上升和随后的下降都更明显。
冬季健康年轻成年人每日补充2000国际单位维生素D可有效。然而，每日4000国际单位对于将水平维持在100纳摩尔/升以上更有效，有助于带来更广泛的健康益处。定期监测[25(OH)D]、钙和磷水平至关重要。
