de Araújo Layanne Guedes Silva, Rodrigues Tigressa Helena Soares, Rates Erick Rafael Dias, Alencar Luciana Magalhães Rebelo, Rosa Morsyleide de Freitas, Ponte Rocha Maria Valderez
Department of Chemical Engineering, Bioengineering and Biomass Valorization Laboratory, Federal University of Ceará, Fortaleza, Ceará 60020-181, Brazil.
Exact Sciences and Technology Center, Chemistry Course, State University of Acaraú Valley, Sobral, Ceará 62040-370, Brazil.
ACS Omega. 2024 Dec 13;9(51):50671-50684. doi: 10.1021/acsomega.4c08702. eCollection 2024 Dec 24.
Cellulose nanostructures obtained from lignocellulosic biomass via enzymatic processes may offer advantages in terms of material properties and processing sustainability. Thus, in this study, cellulose nanoparticles with a spherical morphology were produced through the enzymatic hydrolysis of cashew apple bagasse (CAB). CAB was previously subjected to alkaline and acid-alkali pretreatment, and the pretreated solids were labeled as CAB-PTA and CAB-PT-HA, respectively. The enzymatic hydrolysis was carried out using two different enzymatic loadings (7.5 and 12 FPU/g) of the cellulase complex, and the formation of nanostructures occurred only at 7.5 FPU/g. The results indicated the production of nanocellulose using only CAB-PT-HA as the precursor, obtaining nanosphere structures with a yield of 65.1 ± 2.9% and a diameter range of 57.26-220.66 nm. The nanocellulose showed good thermal and colloidal stability and was subsequently used for biofilm production. Biofilms were prepared using different percentages of nanocellulose (5 and 7% w/v), and they showed a greater water retention capacity and higher biodegradability compared to the control film, indicating potential for application in food packaging and cosmetic masks. Thus, it highlights the potential for developing new biodegradable plastics incorporated with nanocellulose obtained from CAB through a more sustainable process.
通过酶法从木质纤维素生物质中获得的纤维素纳米结构在材料性能和加工可持续性方面可能具有优势。因此，在本研究中，通过对腰果苹果渣（CAB）进行酶水解制备了具有球形形态的纤维素纳米颗粒。CAB先前经过了碱性和酸碱预处理，预处理后的固体分别标记为CAB - PTA和CAB - PT - HA。酶水解使用两种不同酶负载量（7.5和12 FPU/g）的纤维素酶复合物进行，仅在7.5 FPU/g时形成纳米结构。结果表明仅使用CAB - PT - HA作为前体生产出了纳米纤维素，获得了纳米球结构，产率为65.1±2.9%，直径范围为57.26 - 220.66 nm。该纳米纤维素表现出良好的热稳定性和胶体稳定性，随后用于生物膜生产。使用不同百分比（5和7% w/v）的纳米纤维素制备生物膜，与对照膜相比，它们表现出更大的保水能力和更高的生物降解性，表明在食品包装和化妆面膜中的应用潜力。因此，它突出了通过更可持续的工艺开发含有从CAB获得的纳米纤维素的新型可生物降解塑料的潜力。
