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使用质子离子液体预处理腰果果渣:增强酶水解。

Pretreatment of cashew apple bagasse using protic ionic liquids: Enhanced enzymatic hydrolysis.

机构信息

Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Bloco 709, Fortaleza, CE, Brazil.

Embrapa Agroindústria Tropical, Rua Doutora Sara Mesquita, 2270 - Pici, CEP 60511-110 Fortaleza, CE, Brazil.

出版信息

Bioresour Technol. 2017 Jan;224:694-701. doi: 10.1016/j.biortech.2016.11.019. Epub 2016 Nov 8.

DOI:10.1016/j.biortech.2016.11.019
PMID:27864129
Abstract

To enhance the enzymatic digestibility of cashew apple bagasse (CAB) feedstock in order to produce sugar fermentation-derived bioproducts, the CAB was subjected to three different pretreatments with the ionic liquid 2-hydroxyl-ethylammonium acetate (2-HEAA) and characterized by FTIR, NMR and chemical methods. All conditions were able to delignify CAB, however the best lignin removal (95.8%) was achieved through the method performed with 8.7% w/w of CAB/2-HEAA ratio at 130°C for 24h. Although the cellulose crystallinity has been increased in CAB treated with the ionic liquid, but this fact did not influence its digestibility. Nevertheless, the pretreatment with 2-HEAA enhanced significantly the cellulose digestibility, increasing the glucose yield from 48 to 747.72mg/g. Furthermore, 2-HEAA pretreatment was efficient even with reused ionic liquid, obtaining high glucose concentration.

摘要

为了提高腰果废果皮(CAB)饲料的酶解可消化性,以生产糖发酵衍生的生物制品,将 CAB 用离子液体 2-羟乙基铵乙酸盐(2-HEAA)进行了三种不同的预处理,并通过 FTIR、NMR 和化学方法进行了表征。所有条件都能够脱除 CAB 中的木质素,但通过在 130°C 下用 8.7%w/w 的 CAB/2-HEAA 比例处理 24 小时的方法,可实现最好的木质素去除(95.8%)。尽管离子液体处理后的 CAB 的纤维素结晶度有所增加,但这一事实并未影响其可消化性。然而,2-HEAA 的预处理显著提高了纤维素的可消化性,将葡萄糖产率从 48mg/g 提高到 747.72mg/g。此外,即使使用重复使用的离子液体,2-HEAA 预处理也非常有效,可获得高浓度的葡萄糖。

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