Sethi Geetanshu, Sood Shaveta, Bhardwaj Sonia Bhonchal, Jain Ashish
Department of Periodontics, Maharishi Markendeshwar College of Dental Science and Research, Ambala, Haryana, India.
Department of Periodontics, Dr. Harvansh Singh Judge Institute of Dental Sciences and Hospital, Panjab University, Chandigarh, India.
J Indian Soc Periodontol. 2024 May-Jun;28(3):304-311. doi: 10.4103/jisp.jisp_540_23. Epub 2024 Dec 2.
The extracts obtained from the leaves and seeds of (Fenugreek) are effective against various microbial infections. The phytoconstituents of have shown promising effects as anti-diabetics, anti-helmentic, anti-microbial, antifungal, and antipyretic, but its impact on oral pathogens is yet to be established. Therefore, the present study aimed to explore the antimicrobial efficacy of phytoconstituents of as compared to 0.2% chlorhexidine (CHX).
The methanolic extracts of i.e., fenugreek absolute (FA), diosgenin (DIO), and furanone (FU) were used in this study. The antimicrobial efficacy of these extracts was evaluated by testing the minimal inhibitory concentration, minimal bactericidal concentration (MBC), agar well-diffusion assay, colony-forming unit (CFU) count, and also by using confocal laser scanning microscopy (CLSM) against ATCC 25923, ATCC 29212, ATCC 35218, and ATCC 27853.
The results of the study demonstrated that has anti-microbial activity comparable to 0.2% CHX. Well-diffusion assay and CFU count assay of the extracts showed statistically significant ( < 0.001) results. MIC and MBC values were observed for FA, DIO, and FU compared to CHX against these selected test organisms. These results were confirmed by visual validation with CLSM.
The use of herbal alternatives in periodontics might prove to be advantageous. can be used as a promising alternative to CHX against , and for the management of oral and periodontal infections.
胡芦巴的叶和种子提取物对多种微生物感染有效。胡芦巴的植物成分已显示出作为抗糖尿病、抗蠕虫、抗微生物、抗真菌和解热剂的有前景的作用，但其对口腔病原体的影响尚未确定。因此，本研究旨在探讨胡芦巴植物成分与0.2%氯己定（CHX）相比的抗菌效果。
本研究使用了胡芦巴的甲醇提取物，即胡芦巴纯提取物（FA）、薯蓣皂苷元（DIO）和呋喃酮（FU）。通过测试最低抑菌浓度、最低杀菌浓度（MBC）、琼脂孔扩散试验、菌落形成单位（CFU）计数，以及使用共聚焦激光扫描显微镜（CLSM）针对金黄色葡萄球菌ATCC 25923、粪肠球菌ATCC 29212、牙龈卟啉单胞菌ATCC 35218和白色念珠菌ATCC 27853来评估这些提取物的抗菌效果。
研究结果表明，胡芦巴具有与0.2% CHX相当的抗菌活性。提取物的孔扩散试验和CFU计数试验显示出具有统计学意义（P < 0.001）的结果。与CHX相比，观察到了FA、DIO和FU针对这些选定测试微生物的MIC和MBC值。这些结果通过CLSM的视觉验证得到了证实。
在牙周病学中使用草药替代品可能被证明是有利的。胡芦巴可以作为一种有前景的替代CHX的药物，用于对抗金黄色葡萄球菌、粪肠球菌、牙龈卟啉单胞菌和白色念珠菌，以管理口腔和牙周感染。
