Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China.
Faculty of Dentistry, University of Toronto, Toronto, Canada.
Microb Pathog. 2020 Dec;149:104482. doi: 10.1016/j.micpath.2020.104482. Epub 2020 Sep 10.
Enterococcus faecalis is a biofilm-forming, nosocomial pathogen that is frequently isolated from failed root canal treatments. Contemporary root canal disinfectants are ineffective in eliminating these biofilms and preventing reinfection. As a result, there is a pressing need to identify novel and safe antibiofilm molecules. The effect of short-term (5 and 15 min) and long-term (24 h) treatments of trans-cinnamaldehyde (TC) on the viability of E. faecalis biofilms was compared with currently used root canal disinfectants. Treatment for 15 min with TC reduced biofilm metabolic activity as effective as 1% sodium hypochlorite and 2% chlorhexidine. Treatment with TC for 24 h was significantly more effective than 2% chlorhexidine in reducing the viable cell counts of biofilms. This serendipitous effect of TC was sustained for 10 days under growth-favoring conditions. For the first time, our study highlights the strong antibacterial activity of TC against E. faecalis biofilms, and notably, its ability to prevent biofilm recovery after treatment.
粪肠球菌是一种生物膜形成的医院病原体，常从失败的根管治疗中分离出来。目前的根管消毒剂无法有效消除这些生物膜并防止再次感染。因此，迫切需要寻找新的安全的抗生物膜分子。本研究比较了肉桂醛（TC）短期（5 和 15 分钟）和长期（24 小时）处理对粪肠球菌生物膜活力的影响，与目前使用的根管消毒剂进行了比较。用 TC 处理 15 分钟，其降低生物膜代谢活性的效果与 1%次氯酸钠和 2%洗必泰相当。用 TC 处理 24 小时，与 2%洗必泰相比，显著降低生物膜活菌数。在有利于生长的条件下，这种 TC 的偶然作用可持续 10 天。本研究首次强调了 TC 对粪肠球菌生物膜的强大抗菌活性，特别是其在治疗后防止生物膜恢复的能力。
