癌基因：它们在头颈部鳞状细胞癌中的存在及意义。

Oncogenes: their presence and significance in squamous cell cancer of the head and neck.

作者信息

Friedman W H, Rosenblum B N, Loewenstein P, Thornton H, Katsantonis G, Green M

出版信息

Laryngoscope. 1985 Mar;95(3):313-6. doi: 10.1288/00005537-198503000-00015.

DOI:10.1288/00005537-198503000-00015

Abstract

DNA extracted from squamous cell carcinomas of the larynx and tongue has been shown to contain cellular transforming genes characterized by their ability to transform mouse fibroblasts into malignant foci of cells which, when subsequently cloned and grown to volume, have been found to contain human DNA sequences. This DNA has been serially passaged through subsequent populations of NIH/3T3 mouse fibroblasts. Higher malignant transformation efficiencies have been observed and reported with serial passage. Of greater significance is the repeated identification of oncogenes of identical characteristics on electrophoretic radioisotope analysis.

摘要

从喉和舌的鳞状细胞癌中提取的DNA已被证明含有细胞转化基因，其特征在于它们能够将小鼠成纤维细胞转化为恶性细胞灶，当随后进行克隆并生长到一定数量时，已发现含有人类DNA序列。这种DNA已在随后的NIH/3T3小鼠成纤维细胞群体中连续传代。随着连续传代，已观察到并报道了更高的恶性转化效率。更重要的是，在电泳放射性同位素分析中反复鉴定出具有相同特征的癌基因。