Cheng P, Li H, Yang P, Tan Y
Department of Stomatology, Tongji Hospital, Tongji Medical University, Wuhan.
J Tongji Med Univ. 1996;16(1):58-60. doi: 10.1007/BF02889048.
The amplification and the G-T mutation at codon 12 of the C-Ha-ras oncogene in oral leukoplakia tissues were analyzed by using polymerase chain reaction (PCR) and molecular biologic technique. The results showed that these tissues had no amplification of Ha-ras oncogene. Only one case harbored G-T mutation in 11 oral leukoplakias, but this mutation was absent in 10 normal oral mucosal tissues. The possible role and significance of C-Ha-ras oncogene in oral precancerous lesions were also discussed.
采用聚合酶链反应（PCR）和分子生物学技术，对口腔白斑组织中C-Ha-ras癌基因第12密码子的扩增及G-T突变进行了分析。结果显示，这些组织中未出现Ha-ras癌基因的扩增。11例口腔白斑中仅有1例存在G-T突变，而10例正常口腔黏膜组织中未发现该突变。同时还讨论了C-Ha-ras癌基因在口腔癌前病变中的可能作用及意义。
