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表达H9N2禽流感病毒HA蛋白的重组C型禽偏肺病毒稳定且能诱导产生保护作用。

Recombinant avian metapneumovirus subtype C expressing HA protein of H9N2 avian influenza virus are stable and induce protection.

作者信息

Guo Yu, Cheng Jing, Zhang Shuai, Zhang Yuanyuan, Zuo Yuzhu, Liu Tao, Wang Yun, Yang Chun, Cheng Chunjie, Fan Jinghui, Jiang Haijun

机构信息

The College of Veterinary Medicine, Agricultural University of Hebei, Baoding, China.

Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.

出版信息

Front Microbiol. 2024 Dec 18;15:1513474. doi: 10.3389/fmicb.2024.1513474. eCollection 2024.

DOI:10.3389/fmicb.2024.1513474
PMID:39744386
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11688360/
Abstract

To prevent H9N2 avian influenza virus (AIV) and Avian metapneumonovirus/C (aMPV/C) infections, we constructed recombinant aMPV/C viruses expressing the HA protein of H9N2 AIV. In addition, EGFP was inserted into the intermediate non-coding region of P-M protein in the aMPV/C genome using a reverse genetic system. The conditions for rescuing the recombinant virus were enhanced followed by insertion of the H9N2 AIV HA gene into the same location in the aMPV/C. The constructed recombinant virus raMPV/C-HA expressed the H9N2 AIV HA protein and showed good stability. Immunization of chicks with raMPV/C-HA increased the generation of neutralizing antibodies against aMPV and H9N2 AIV for 21 days. In the late challenge experiment, raMPV/C-HA effectively inhibited the replication of the virus , decreased the incidence of infection and conferred protection effects.

摘要

为预防H9N2禽流感病毒（AIV）和禽偏肺病毒/C型（aMPV/C）感染，我们构建了表达H9N2 AIV血凝素（HA）蛋白的重组aMPV/C病毒。此外，利用反向遗传系统将绿色荧光蛋白（EGFP）插入aMPV/C基因组中P-M蛋白的中间非编码区。在将H9N2 AIV HA基因插入aMPV/C的相同位置后，优化了重组病毒拯救条件。构建的重组病毒raMPV/C-HA表达H9N2 AIV HA蛋白并表现出良好的稳定性。用raMPV/C-HA免疫雏鸡可在21天内增加针对aMPV和H9N2 AIV的中和抗体产生。在后期攻毒实验中，raMPV/C-HA有效抑制病毒复制，降低感染发生率并具有保护作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3219/11688360/f87a0c6bb3f9/fmicb-15-1513474-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3219/11688360/85e6beb1086a/fmicb-15-1513474-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3219/11688360/eac8273e0d28/fmicb-15-1513474-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3219/11688360/79ed51b58aca/fmicb-15-1513474-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3219/11688360/5ba016b092a7/fmicb-15-1513474-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3219/11688360/d579123992f6/fmicb-15-1513474-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3219/11688360/f87a0c6bb3f9/fmicb-15-1513474-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3219/11688360/85e6beb1086a/fmicb-15-1513474-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3219/11688360/eac8273e0d28/fmicb-15-1513474-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3219/11688360/79ed51b58aca/fmicb-15-1513474-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3219/11688360/5ba016b092a7/fmicb-15-1513474-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3219/11688360/d579123992f6/fmicb-15-1513474-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3219/11688360/f87a0c6bb3f9/fmicb-15-1513474-g006.jpg

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