一种新型冠状病毒2型快速抗原检测与实时荧光定量聚合酶链反应的比较
Comparison of a Commercial SARS-CoV-2 Rapid Antigen Test with Real-Time PCR.
作者信息
Yıldız Muhammet Çağrı, Ağca Harun, Ardıç Anıl, Sağlık İmran, Ener Beyza
机构信息
Department of Medical Microbiology, Bursa Uludağ University School of Medicine, Bursa, Türkiye.
Department of Emergency Medicine, Bursa Uludağ University School of Medicine, Bursa, Türkiye.
出版信息
Infect Dis Clin Microbiol. 2024 Dec 19;6(4):298-305. doi: 10.36519/idcm.2024.421. eCollection 2024 Dec.
OBJECTIVE
The study compared the mö-screen Corona Antigen Test (Qiagen, Germany) with RT-PCR in suspected COVID-19 patients.
MATERIALS AND METHODS
Two hundred combined oro-nasopharyngeal swabs were collected from patients with suspected COVID-19 to evaluate the analytical performance of the mö-screen Corona Antigen Test compared to qualitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) in symptomatic patients.
RESULTS
The mö-screen Corona Antigen Test showed an overall agreement with a sensitivity of 100%, a specificity of 100%, a positive predictive value (PPV) of 100%, and a negative predictive value (NPV) of 100%. We semi-quantitatively evaluated the mö-screen Corona Antigen Test and found a -0.706 correlation between cycle threshold (Ct) values and antigen test results (<0.001).
CONCLUSION
The accuracy achievable by the mö-screen Corona Antigen Test, combined with the rapid turnaround time compared to RT-PCR, suggests that rapid antigen tests can be used for rapid diagnosis.
目的
本研究比较了疑似新型冠状病毒肺炎（COVID-19）患者中mö-screen冠状病毒抗原检测（德国Qiagen公司）与逆转录聚合酶链反应（RT-PCR）的效果。
材料与方法
从疑似COVID-19患者中采集200份联合口咽拭子和鼻咽拭子，以评估mö-screen冠状病毒抗原检测相对于有症状患者的定性实时逆转录聚合酶链反应（RT-PCR）的分析性能。
结果
mö-screen冠状病毒抗原检测总体一致性良好，灵敏度为100%，特异性为100%，阳性预测值（PPV）为100%，阴性预测值（NPV）为100%。我们对mö-screen冠状病毒抗原检测进行了半定量评估，发现循环阈值（Ct）值与抗原检测结果之间的相关性为-0.706（<0.001）。
结论
mö-screen冠状病毒抗原检测可达到的准确性，再加上与RT-PCR相比周转时间短，表明快速抗原检测可用于快速诊断。
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