Saju Adeela Fathima, Mukundan Aditi, Chandrashekhar Raghu, Mahadev Rao Archana
Department of Biotechnology, Manipal Institute of Technology (MIT), Manipal Academy of Higher Education (MAHE), Manipal, India.
Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences (MCOPS), Manipal Academy of Higher Education (MAHE), Manipal, India.
RNA Biol. 2025 Dec;22(1):1-11. doi: 10.1080/15476286.2024.2449277. Epub 2025 Jan 3.
RNA-focused therapy and diagnostics have been making waves in molecular biology due to the advantages RNA has over DNA; for instance, the ability of RNA to target nearly any genetic component in the cell is a big step in treating disorders. Moreover, RNA-based diagnosis of diseases is only becoming increasingly popular, especially after the COVID-19 pandemic, which brought up the need for cost-effective and efficient diagnosing kits for the vast majority. RNA-based techniques also have close to no risk of genotoxicity and can efficiently target undruggable regions of the cell. RNA treatments have effectively shown the future of the medical industry in the past couple of decades, and they will only be seen to improve. This review paper provides an overview on the different techniques that use RNA-based approaches in the field of diagnostics and therapeutics.
由于RNA相对于DNA具有诸多优势，聚焦于RNA的治疗和诊断在分子生物学领域掀起了波澜；例如，RNA能够靶向细胞内几乎任何遗传成分，这在治疗疾病方面迈出了重要一步。此外，基于RNA的疾病诊断正变得越来越流行，尤其是在新冠疫情之后，这使得绝大多数人对经济高效的诊断试剂盒的需求大增。基于RNA的技术几乎没有基因毒性风险，并且能够有效地靶向细胞中难以成药的区域。在过去几十年里，RNA治疗已切实展现了医疗行业的未来，而且只会越来越好。这篇综述文章概述了诊断和治疗领域中使用基于RNA的方法的不同技术。
