从用于从褐藻生产海藻肥料的[具体物种名称]中定向进化出一种藻酸盐裂解酶。
Directed Evolution of an Alginate Lyase from sp. for Seaweed Fertilizer Production from the Brown Seaweed .
作者信息
Jiang Jun, Hu Zhiyi, Wang Yue, Jiang Zhengqiang, Yan Qiaojuan, Yang Shaoqing
机构信息
College of Food Science and Nutritional Engineering, China Agricultural University, No.17 Qinghua East Road, Haidian District, Beijing 100083, China.
Key Laboratory of Food Bioengineering (China National Light Industry), College of Engineering, China Agricultural University, No.17 Qinghua East Road, Haidian District, Beijing 100083, China.
出版信息
J Agric Food Chem. 2025 Jan 15;73(2):1468-1477. doi: 10.1021/acs.jafc.4c09016. Epub 2025 Jan 2.
An alginate lyase (FsAly7) from sp. was engineered by directed evolution to improve its optimum temperature and thermostability. The optimum temperature of the positive mutant mFsAly7 (FsAly7-Ser43Pro) was increased by 5 °C, and the thermal inactivation half-lives at 40 and 45 °C were 4.4 and 5.6 times higher than those of FsAly7, respectively. mFsAly7 was expressed in high levels in with the highest yield of 3125.5 U·mL. On the property-improved enzyme, a bioconversion strategy was developed to prepare hydrolysate, and the highest AOS yield (29.5 mg·mL) was 1.5 times higher than that of FsAly7. Moreover, the application potential of the hydrolysate as an organic fertilizer was evaluated. The addition of hydrolysate improved the sprouting rate, fresh mass, seedling height, and stem diameter of the buckwheat sprouts. Therefore, mFsAly7 may be a good candidate for the production of seaweed fertilizers from .
通过定向进化对来自[具体物种]的一种海藻酸裂解酶（FsAly7）进行改造，以提高其最适温度和热稳定性。阳性突变体mFsAly7（FsAly7-Ser43Pro）的最适温度提高了5℃，在40℃和45℃下的热失活半衰期分别比FsAly7高4.4倍和5.6倍。mFsAly7在[具体表达宿主]中高水平表达，最高产量为3125.5 U·mL。基于这种性质改良的酶，开发了一种生物转化策略来制备[具体海藻酸]水解产物，最高的AOS产量（29.5 mg·mL）比FsAly7高1.5倍。此外，还评估了[具体海藻酸]水解产物作为有机肥料的应用潜力。添加[具体海藻酸]水解产物提高了苦荞芽的发芽率、鲜重、苗高和茎粗。因此，mFsAly7可能是利用[具体海藻]生产海藻肥料的良好候选酶。
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