长链非编码RNA G2E3-AS1和BACE1-AS的上调作为转移性结直肠癌的预后生物标志物

Upregulation of LncRNAs G2E3-AS1 and BACE1-AS as prognostic biomarkers in metastatic colorectal cancer.

作者信息

Kenari Shahrbanoo Nandoust, Mohamadynejad Parisa, Moghanibashi Mehdi, Bagheri Abouzar, Rouhi Leila

机构信息

Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.

Department of Genetics, Faculty of Basic Sciences, Kazerun Branch, Islamic Azad University, Kazerun, Iran.

出版信息

Biomarkers. 2025 Feb;30(1):88-96. doi: 10.1080/1354750X.2024.2448508. Epub 2025 Jan 30.

DOI:10.1080/1354750X.2024.2448508

PMID:39745049

Abstract

BACKGROUND

Despite the current diagnostic and therapeutic methods for colorectal cancer (CRC), patients are often diagnosed at advanced stages of colorectal cancer. Recently, numerous investigations have highlighted the role of lncRNAs in cancer development and progression. This study investigated less well-characterized genes in the colorectal cancer metastasis process.

MATERIALS AND METHODS

Genes expression profiles from CRC patients were downloaded from the TCGA database by the TCGAbiolinks R package. Differential gene expression analysis of miRNA, lncRNAs, and mRNAs was conducted for the M1 and M0 compared to control samples. Then, the DIANA lncbase3 tool was used to find M1-specific miRNA-LncRNA interactions. In addition, the expression of selected genes was evaluated by Real-time RT-PCR in forty-one CRC tissues.

RESULTS

Our analysis showed that the expression levels of 77 lncRNAs, 12 miRNAs, and 627 mRNA were significantly changed only in metastatic tumors. In experimental study, significant overexpression of LncRNAs LINC00839, LINC01006, BACE1-AS and G2E3-AS1 was confirmed in metastatic tumors. Also, ROC analysis showed that these lncRNAs, especially lncRNAs G2E3-AS1 and BACE1-AS, are good prognostic biomarkers for metastatic colorectal tumors.

CONCLUSION

We demonstrated that the lncRNAs G2E3-AS1 and BACE1-AS expression upregulated in CRC tissues can be good potential biomarkers for metastatic colorectal cancer.

摘要

背景

尽管目前存在针对结直肠癌（CRC）的诊断和治疗方法，但患者常被诊断为结直肠癌晚期。最近，大量研究突出了长链非编码RNA（lncRNAs）在癌症发生和发展中的作用。本研究调查了结直肠癌转移过程中特征尚不明确的基因。

材料与方法

通过TCGAbiolinks R包从TCGA数据库下载CRC患者的基因表达谱。将M1和M0样本与对照样本进行比较，对miRNA、lncRNAs和mRNA进行差异基因表达分析。然后，使用DIANA lncbase3工具查找M1特异性miRNA-LncRNA相互作用。此外，通过实时逆转录聚合酶链反应（Real-time RT-PCR）评估41例CRC组织中所选基因的表达。

结果

我们的分析表明，仅在转移性肿瘤中77种lncRNAs、12种miRNAs和627种mRNA的表达水平发生了显著变化。在实验研究中，转移性肿瘤中LncRNAs LINC00839、LINC01006、BACE1-AS和G2E3-AS1的表达被证实显著上调。此外，ROC分析表明，这些lncRNAs，尤其是lncRNAs G2E3-AS1和BACE1-AS，是转移性结直肠肿瘤的良好预后生物标志物。