Zhang Geng, Ichikawa Kenta, Iitani Kenta, Iwasaki Yasuhiko, Mitsubayashi Kohji
Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
Department of Biomedical Devices and Instrumentation, Laboratory for Biomaterials and Bioengineering, Institute of Integrated Research, Institute of Science Tokyo, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Analyst. 2025 Jan 27;150(3):505-512. doi: 10.1039/d4an01281j.
As a marker of human metabolism, acetone is important for lipid metabolism monitoring and early detection of diabetes. In this study, we developed a handheld biosensor for acetone based on fluorescence detection by utilizing the enzymatic reaction of secondary alcohol dehydrogenase (S-ADH) with β-nicotinamide adenine dinucleotide (NADH, = 340 nm, = 490 nm). In the reaction, NADH is oxidized when acetone is reduced to 2-propanol by S-ADH, and the acetone concentration can be measured by detecting the amount of NADH consumed in this reaction. First, we constructed a compact and light-weight fluorometric NADH detection system (209 g for the sensing system and 342 g for the PC), which worked using battery power. Then, sensor characteristics were evaluated after optimization of the working conditions. The developed system was able to quantify acetone in a range of 510 nM-1 mM within 1 minute. The developed battery-operated acetone biosensor demonstrated its ability to measure the acetone concentration in the exhaled breath condensate of 10 healthy subjects at rest (23.4 ± 15.1 μM) and after 16 h of fasting (37.7 ± 14.7 μM) and it distinguished the results with significant differences ( = 0.011). With the advantages of handheld portability, and high levels of sensitivity and selectivity, this sensor is expected to be widely used in clinical diagnosis and wearable biochemical sensors in the future.
作为人体新陈代谢的标志物，丙酮对于脂质代谢监测和糖尿病的早期检测具有重要意义。在本研究中，我们基于荧光检测开发了一种用于丙酮的手持式生物传感器，该传感器利用仲醇脱氢酶（S-ADH）与β-烟酰胺腺嘌呤二核苷酸（NADH， = 340 nm， = 490 nm）的酶促反应。在该反应中，当丙酮被S-ADH还原为2-丙醇时，NADH被氧化，通过检测该反应中消耗的NADH量可测定丙酮浓度。首先，我们构建了一个紧凑且轻便的荧光NADH检测系统（传感系统重209 g，电脑重342 g），该系统使用电池供电。然后，在优化工作条件后评估传感器特性。所开发的系统能够在1分钟内对510 nM - 1 mM范围内的丙酮进行定量。所开发的电池供电的丙酮生物传感器展示了其测量10名健康受试者静息时（23.4 ± 15.1 μM）和禁食16小时后（37.7 ± 14.7 μM）呼出气冷凝物中丙酮浓度的能力，且区分结果具有显著差异（ = 0.011）。凭借手持式便携性、高灵敏度和高选择性等优点，该传感器有望在未来广泛应用于临床诊断和可穿戴生化传感器。
