Iitani Kenta, Ishizuki Naohiro, Matsuhashi Yuka, Yokota Kumi, Ichikawa Kenta, Toma Koji, Arakawa Takahiro, Iwasaki Yasuhiko, Mitsubayashi Kohji
Department of Biomedical Devices and Instrumentation, Laboratory for Biomaterials and Bioengineering, Institute of Integrated Research, Institute of Science Tokyo, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
Anal Chem. 2024 Dec 24;96(51):20197-20203. doi: 10.1021/acs.analchem.4c03816. Epub 2024 Dec 13.
Acetone gas in exhaled breath and skin gas is produced when fatty acids are used as an energy source in the body. The selective and sensitive continuous measurement of acetone gas would be useful for the early screening of diabetes mellitus, a condition characterized by increased fatty acid metabolism. In particular, there is a growing need for acetone gas sensors that enable the wearable measurement of trace concentrations of acetone gas emitted through the skin. In this study, we improved the sensor's sensitivity by optimizing the fluorescence measurement system and flow cell of a previously developed biofluorometric acetone gas sensor (bio-sniffer) using secondary alcohol dehydrogenase. To evaluate the performance of the improved acetone bio-sniffer, we constructed a dilution system to prepare acetone gas at subparts per billion by volume (ppbv) levels and verified its accuracy using gas chromatography-mass spectrometry. As a result, the dilution system was able to prepare 0.1 ppbv of acetone gas with a coefficient of variation of 5%. The improved acetone bio-sniffer demonstrated quantitative characteristics in the concentration range of 0.5-1000 ppbv, with a lower limit of quantification that was 40 times better than that of the conventional acetone bio-sniffer. In the future, this system is expected to be used for continuous measurement of acetone gas released through the skin.
当身体将脂肪酸用作能量来源时，呼出气体和皮肤气体中会产生丙酮气体。对丙酮气体进行选择性和灵敏的连续测量，将有助于糖尿病的早期筛查，糖尿病是一种以脂肪酸代谢增加为特征的疾病。特别是，对能够可穿戴测量通过皮肤散发的痕量丙酮气体浓度的传感器的需求日益增长。在本研究中，我们通过优化先前开发的使用仲醇脱氢酶的生物荧光丙酮气体传感器（生物嗅探器）的荧光测量系统和流通池，提高了传感器的灵敏度。为了评估改进后的丙酮生物嗅探器的性能，我们构建了一个稀释系统，以制备体积分数为十亿分之几（ppbv）水平的丙酮气体，并使用气相色谱-质谱法验证其准确性。结果，该稀释系统能够制备0.1 ppbv的丙酮气体，变异系数为5%。改进后的丙酮生物嗅探器在0.5-1000 ppbv的浓度范围内表现出定量特性，其定量下限比传统丙酮生物嗅探器提高了40倍。未来，该系统有望用于连续测量通过皮肤释放的丙酮气体。
