Aras Serhat, Ozkanli Seyma, Sumer Engin, Koprulu Tugba Kul, Efendioglu Mustafa
Department of Radiation Oncology, Haydarpasa Numune Training and Research Hospital, University of Health Sciences, Uskudar, Istanbul, Turkey.
Medical Imaging Techniques, Department of Medical Services and Techniques, Hamidiye Vocational School of Health Services, University of Health Sciences, Uskudar, Istanbul, Turkey.
Int J Radiat Biol. 2025;101(3):264-273. doi: 10.1080/09553002.2024.2445582. Epub 2025 Jan 2.
The aim of this study was to investigate the radiobiological effects underlying the inhibition of breast cancer (BCa) following radiotherapy in nude mice models, and to evaluate the impact of changes in immunohistochemical parameters induced by FF and FFF beams.
The study included thirty-six adult nude mouse models, which were randomly assigned to five groups: control (G1), breast cancer (BCa) (G2), FF-400 MU/min (G3), FFF-1100 MU/min (G4), and FFF-1800 MU/min (G5). The control group received neither radiation nor treatment, while the BCa group had a cancer model without radiation. The BCa models were subjected to a single dose of 20 Gy of radiotherapy at varying dose rates. Twenty days after the implantation of the MCF-7 cancer cell line, the nude mice were irradiated and sacrificed 48 h later for ER, PR, HER-2, Ki-67, CD-133, Caspase-3, APAF-1, NOS-2 and NOS-3 IHC analysis.
A statistically significant decrease in IHC staining values for ER, Ki-67 and NOS-2 was observed in the FF-400 MU/min, FFF-1100 MU/min and FFF-1800 MU/min groups due to radiotherapy compared to the BCa group. The FFF beams demonstrated superior efficacy in the treatment of BCa. The significant differences in Caspase-3 and APAF-1 levels were found between BCa and control groups, while CD-133, NOS-3, HER-2, and PR staining showed no differences between groups.
It was concluded that FFF beam was more effective than FF beam for BCa, especially on ER, Ki-67 and NOS-2 IHC parameters.
本研究旨在探讨裸鼠模型中放射治疗后乳腺癌（BCa）抑制背后的放射生物学效应，并评估FF和FFF线束诱导的免疫组化参数变化的影响。
本研究纳入36只成年裸鼠模型，随机分为五组：对照组（G1）、乳腺癌（BCa）组（G2）、FF - 400 MU/分钟组（G3）、FFF - 1100 MU/分钟组（G4）和FFF - 1800 MU/分钟组（G5）。对照组既不接受放疗也不接受治疗，而BCa组有未接受放疗的癌症模型。对BCa模型以不同剂量率给予单次20 Gy的放射治疗。在植入MCF - 7癌细胞系20天后，对裸鼠进行照射，并在48小时后处死，进行雌激素受体（ER）、孕激素受体（PR）、人表皮生长因子受体2（HER - 2）、细胞增殖核抗原（Ki - 67）、CD - 133、半胱天冬酶 - 3（Caspase - 3）、凋亡蛋白酶激活因子 - 1（APAF - 1）、一氧化氮合酶 - 2（NOS - 2）和一氧化氮合酶 - 3（NOS - 3）免疫组化分析。
与BCa组相比，放疗后FF - 400 MU/分钟组、FFF - 1100 MU/分钟组和FFF - 1800 MU/分钟组的ER、Ki - 67和NOS - 2免疫组化染色值出现统计学显著下降。FFF线束在BCa治疗中显示出更高的疗效。BCa组和对照组之间Caspase - 3和APAF - 1水平存在显著差异，而CD - 133、NOS - 3、HER - 2和PR染色在各组之间无差异。
得出结论，FFF线束对BCa比FF线束更有效，尤其是对ER、Ki - 67和NOS - 2免疫组化参数。
