Liao Yonghe, Yi Tingzhuang, Tan Kangyi, Su Xiaoyan, Chen Shuixiu, Lu Mingyue, Yang Yanchun, Huang Xianxian, Zhao Yiheng, Huang Hong, Jiang Neng, Yan Zhiming, Wei Changhong
Department of Research & Clinical Laboratory, The Fifth Affiliated Hospital of Guangxi Medical University, The First People's Hospital of Nanning, Nanning 530022, Guangxi, PR China; Pharmaceutical College, Guangxi Medical University, Nanning 530021, Guangxi, PR China.
Department of Oncology, Affiliated Hospital of Youjiang Medical University For Nationalities, Guangxi Clinical Medical Research Center for Hepatobiliary Diseases, Baise 533000, Guangxi, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Apr 5;330:125680. doi: 10.1016/j.saa.2024.125680. Epub 2024 Dec 27.
Hypochlorous acid (HClO) represents a typical endogenous reactive oxygen species primarily generated within mitochondria, with excessive levels intimately linked to numerous pathological conditions. Consequently, the detection of mitochondrial HClO in vivo holds significant importance in the biomedical realm. Near-infrared fluorescence (NIRF)/photoacoustic (PA) dual-modality imaging techniques offer enhanced accuracy in biological imaging. Herein, a novel mitochondria-targeted NIRF/PA dual-modality probe (MB-ClO) has been designed and synthesized. MB-ClO exhibits remarkable selectivity, ultra-sensitivity (LOD = 5.1 nM) towards HClO, coupled with a swift fluorescence response (<10 s). MB-ClO possesses low toxicity and excellent water solubility, making it suitable for biological applications. Moreover, MB-ClO is capable of targeting mitochondria. MB-ClO has been successfully utilized to detect HClO in biological systems. In addition, MB-ClO has been utilized for NIRF/PA dual-modality imaging in a mouse model of rheumatoid arthritis, demonstrating its extensive application potential and value in biomedical research.
