School of Pharmacy, Yancheng Teachers' University, Yancheng, Jiangsu 224051, People's Republic of China.
School of Pharmacy, Yancheng Teachers' University, Yancheng, Jiangsu 224051, People's Republic of China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Mar 5;228:117825. doi: 10.1016/j.saa.2019.117825. Epub 2019 Nov 19.
Hypochlorous/hypochlorite (HClO/ClO), one of the most important signal molecule, plays a crucial role in many cellular signaling pathways. It is reported that the HClO/ClO level in mitochondria is important to maintain the normal mitochondrial function. Herein, we present two simple fluorescent probes BAC and mitochondria-targeting fluorescent probe TACB for the detection of ClO. Probes BAC &TACB could be sensitively and selectivity detecting ClO at the nanomolar levels with the detection limit of 1.64 × 10 M and 9.86 × 10 M, respectively. Additionally, probes BAC &TACB with the response unit of CO moiety could selectively detect ClO over other various analytes such as anions, metal ions and OH, O, HO. The response time of probe TACB for ClO (<20 s), implying that it could offer a real-time analytical assay of ClO. Finally, probe BAC was used for monitoring the ClO in HEK293T cells and probe TACB could be utilized to track the fluctuations of exogenous ClO levels in the mitochondria of Hela cells.
次氯酸/次氯酸盐（HClO/ClO）作为最重要的信号分子之一，在许多细胞信号通路中起着至关重要的作用。据报道，线粒体中的 HClO/ClO 水平对于维持正常的线粒体功能很重要。在此，我们提出了两种简单的荧光探针 BAC 和线粒体靶向荧光探针 TACB 来检测 ClO。探针 BAC 和 TACB 可以在纳摩尔水平上灵敏且选择性地检测 ClO，检测限分别为 1.64×10-7 M 和 9.86×10-7 M。此外，具有 CO 部分反应单元的探针 BAC 和 TACB 可以选择性地检测 ClO 而不是其他各种分析物，如阴离子、金属离子和 OH、O、HO。探针 TACB 对 ClO 的响应时间（<20 s）表明它可以提供 ClO 的实时分析测定。最后，探针 BAC 用于监测 HEK293T 细胞中的 ClO，探针 TACB 可用于跟踪 Hela 细胞线粒体中外源 ClO 水平的波动。
