Wu Ke, Vu Emily D, Ghosh Saptarshi, Mishra Ruchir, Bonning Bryony C
Department of Entomology and Nematology, University of Florida, Gainesville, FL, USA.
University of Florida Genetics Institute, Gainesville, FL, USA.
Sci Rep. 2025 Jan 2;15(1):124. doi: 10.1038/s41598-024-83671-2.
The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), is a major pest of global citriculture. In the Americas and in Asia, D. citri vectors the phloem-limited bacterium, Candidatus Liberibacter asiaticus (CLas), which causes the fatal citrus disease huanglongbing, or citrus greening. Cell lines derived from D. citri can provide insight into both the basic biology of this pest and D. citri-associated pathogens including CLas. We previously identified CLG#2 as the optimal medium for long-term growth of D. citri primary cell cultures. Here we report on the establishment and characterization of three continuous D. citri cell lines, Dici1, Dici3, and Dici5, that have been passaged for > 40 times. Based on morphological and transcriptomic data, the Dici1 and Dici3 cell lines include undifferentiated and neurogenic progenitor cells. Dici1 and Dici5 are infected with Wolbachia. Both Dici1 and Dici5 are infected with D. citri reovirus, and Dici5 is also infected with D. citri-associated C virus. Dici3 is free of both Wolbachia and virus infection. These cell lines provide an ideal platform for the study of inter-microbial relationships as well as microbe interaction with host insect cells.
亚洲柑橘木虱，Diaphorina citri Kuwayama（半翅目：木虱科），是全球柑橘种植业的主要害虫。在美洲和亚洲，亚洲柑橘木虱传播韧皮部限制细菌“亚洲韧皮杆菌”（CLas），该细菌会引发致命的柑橘黄龙病，即柑橘绿变病。源自亚洲柑橘木虱的细胞系能够为了解这种害虫的基础生物学特性以及包括CLas在内的与亚洲柑橘木虱相关的病原体提供见解。我们之前确定CLG#2是亚洲柑橘木虱原代细胞培养物长期生长的最佳培养基。在此，我们报告了三个连续传代超过40次的亚洲柑橘木虱细胞系Dici1、Dici3和Dici5的建立及特性。基于形态学和转录组数据，Dici1和Dici3细胞系包含未分化的神经源性祖细胞。Dici1和Dici5感染了沃尔巴克氏体。Dici1和Dici5均感染了亚洲柑橘木虱呼肠孤病毒，并且Dici5还感染了与亚洲柑橘木虱相关的C病毒。Dici3未感染沃尔巴克氏体和病毒。这些细胞系为研究微生物间关系以及微生物与宿主昆虫细胞的相互作用提供了理想平台。
