Liu Luqin, Chen Jing, Jiang Junyao, Liang Jiamei, Song Yaqin, Chen Qi, Yan Fuling, Bai Ziqin, Song Zhen, Liu Jinxiang
Citrus Research Institute, Southwest University/National Citrus Engineering Research Center, Chongqing, China.
Guangxi Academy of Specialty Crops, Guangxi Citrus Breeding and Cultivation Research Center of Engineering Technology, Guangxi, China.
Front Plant Sci. 2024 Feb 6;15:1357163. doi: 10.3389/fpls.2024.1357163. eCollection 2024.
Asian citrus psyllid (ACP, ) is an important transmission vector of " Liberibacter asiaticus" (Las), the causal agent of Huanglongbing (HLB), the most destructive citrus disease in the world. As there are currently no HLB-resistant rootstocks or varieties, the control of ACP is an important way to prevent HLB. Some viruses of insect vectors can be used as genetically engineered materials to control insect vectors.
To gain knowledge on viruses in ACP in China, the prevalence of five RNA and DNA viruses was successfully determined by optimizing reverse transcription polymerase chain reaction (RT-PCR) in individual adult ACPs. The five ACP-associated viruses were identified as follows: diaphorina citri bunyavirus 2, which was newly identified by high-throughput sequencing in our lab, diaphorina citri reovirus (DcRV), diaphorina citri picorna-like virus (DcPLV), diaphorina citri bunyavirus (DcBV), and diaphorina citri densovirus-like virus (DcDV).
DcPLV was the most prevalent and widespread ACP-associated virus, followed by DcBV, and it was detected in more than 50% of all samples tested. DcPLV was also demonstrated to propagate vertically and found more in salivary glands among different tissues. Approximately 60% of all adult insect samples were co-infected with more than one insect pathogen, including the five ACP-associated viruses and Las.
This is the first time these viruses, including the newly identified ACP-associated virus, have been detected in individual adult ACPs from natural populations in China's five major citrus-producing provinces. These results provide valuable information about the prevalence of ACP-associated viruses in China, some of which have the potential to be used as biocontrol agents. In addition, analysis of the change in prevalence of pathogens in a single insect vector is the basis for understanding the interactions between Las, ACP, and insect viruses.
亚洲柑橘木虱（ACP）是“亚洲韧皮杆菌”（Las）的重要传播媒介，Las是黄龙病（HLB）的病原体，HLB是世界上最具破坏性的柑橘病害。由于目前尚无抗HLB的砧木或品种，控制ACP是预防HLB的重要途径。昆虫传播媒介的一些病毒可作为基因工程材料用于控制昆虫传播媒介。
为了解中国ACP体内的病毒情况，通过优化单个成年ACP的逆转录聚合酶链反应（RT-PCR），成功测定了5种RNA和DNA病毒的流行情况。鉴定出的5种与ACP相关的病毒如下：柑橘木虱布尼亚病毒2，这是我们实验室通过高通量测序新鉴定出的；柑橘木虱呼肠孤病毒（DcRV）、柑橘木虱类细小核糖核酸病毒（DcPLV）、柑橘木虱布尼亚病毒（DcBV）和柑橘木虱浓核病毒样病毒（DcDV）。
DcPLV是最普遍且分布最广的与ACP相关的病毒，其次是DcBV，在所有检测样本中超过50%的样本中被检测到。DcPLV还被证明可垂直传播，且在不同组织的唾液腺中含量更高。所有成年昆虫样本中约60%被一种以上昆虫病原体共同感染，包括这5种与ACP相关的病毒和Las。
这是首次在中国五个主要柑橘生产省份的自然种群中的单个成年ACP中检测到这些病毒，包括新鉴定出的与ACP相关的病毒。这些结果提供了有关中国与ACP相关病毒流行情况的有价值信息，其中一些有可能用作生物防治剂。此外，分析单个昆虫传播媒介中病原体流行率的变化是理解Las、ACP和昆虫病毒之间相互作用的基础。
