Mishra Ruchir, Hua Gang, Bagal Ujwal R, Champagne Donald E, Adang Michael J
Department of Entomology, College of Agricultural and Environmental Sciences, University of Georgia, Athens, Georgia, USA.
Department of Entomology and Nematology, University of Florida, Gainesville, Florida, USA.
Insect Sci. 2022 Oct;29(5):1346-1360. doi: 10.1111/1744-7917.13036. Epub 2022 May 9.
Anopheles gambiae and Anopheles coluzzii are closely related species that are predominant vectors of malaria in Africa. Recently, A. gambiae form M was renamed A. coluzzii and we now conclude on the basis of a diagnostic PCR-restriction fragment length polymorphism assay that Ag55 cells were derived from A. coluzzii. We established an Ag55 cell transcriptome, and KEGG pathway analysis showed that Ag55 cells are enriched in phagosome pathway transcripts. The Ag55 transcriptome has an abundance of specific transcripts characteristic of mosquito hemocytes. Functional E. coli bioparticle uptake experiments visualized by fluorescence microscopy and confocal microscopy and quantified by flow cytometry establish the phagocytic competence of Ag55 cells. Results from this investigation of Ag55 cell properties will guide researchers in the use and engineering of the Ag55 cell line to better enable investigations of Plasmodium, other microbes, and insecticidal toxins.
冈比亚按蚊和科氏按蚊是亲缘关系密切的物种，它们是非洲疟疾的主要传播媒介。最近，冈比亚按蚊M型被重新命名为科氏按蚊，我们现在基于诊断性聚合酶链反应-限制性片段长度多态性分析得出结论，Ag55细胞源自科氏按蚊。我们建立了Ag55细胞转录组，KEGG通路分析表明，Ag55细胞富含吞噬体通路转录本。Ag55转录组有大量蚊子血细胞特有的特定转录本。通过荧光显微镜和共聚焦显微镜观察并用流式细胞术定量的功能性大肠杆菌生物颗粒摄取实验确定了Ag55细胞的吞噬能力。对Ag55细胞特性的这项研究结果将指导研究人员使用和改造Ag55细胞系，以便更好地开展对疟原虫、其他微生物和杀虫毒素的研究。
