Wongsamart Rungdawan, Somboonna Naraporn, Cheibchalard Thanya, Klankeo Piriya, Ruampatana Jakavat, Nuntapaitoon Morakot
Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Microbiome Research Unit for Probiotics in Food and Cosmetics, Chulalongkorn University, Bangkok, 10330, Thailand.
Sci Rep. 2025 Jan 2;15(1):17. doi: 10.1038/s41598-024-84573-z.
Maintaining a diverse and balanced sow milk microbiome is essential to piglet development. Thus, this study aimed to examine the effects of probiotic Bacillus licheniformis supplementation on the microbiome composition of sow colostrum and milk, and to review associated health findings in piglets. B. licheniformis DSMZ 28710 was supplemented at 10 g/day as feed additive before predicted farrowing until weaning by top dressing. Colostrum and milk samples were collected for metagenomic DNA extraction, 16s rRNA sequencing, and bioinformatics analyses for bacterial microbiota diversity. Results indicated that the supplementation increased the abundances of beneficial bacteria, such as Lactobacillus, Pediococcus, Bacteroides, and Bifidobacterium, while decreasing the abundances of pathogenic bacteria, such as Staphylococcus aureus, Enterobacteriaceae, and Campylobacter in the colostrum. The supplementation increased diversity while maintaining richness and evenness. Moreover, the rise in predicted microbial community metabolic function in membrane transport pathways provides crucial evidence showing that the supplementation is potentially beneficial to piglets, as these pathways are important for providing nutrients and immunity to offspring. This research highlights the importance of microbiome composition in sow milk and the potential of B. licheniformis supplementation as a means to improve piglet health and development.
维持母猪乳汁中微生物群落的多样性和平衡对仔猪发育至关重要。因此，本研究旨在探讨添加益生菌地衣芽孢杆菌对母猪初乳和乳汁微生物群落组成的影响，并综述仔猪相关的健康研究结果。在预计分娩前至断奶期间，通过顶层添加的方式，将地衣芽孢杆菌DSMZ 28710作为饲料添加剂，以每天10克的剂量添加。采集初乳和乳汁样本用于宏基因组DNA提取、16S rRNA测序以及细菌微生物群多样性的生物信息学分析。结果表明，添加该益生菌增加了有益菌的丰度，如乳酸杆菌、片球菌、拟杆菌和双歧杆菌，同时降低了初乳中病原菌的丰度，如金黄色葡萄球菌、肠杆菌科和弯曲杆菌。添加该益生菌增加了多样性，同时维持了丰富度和均匀度。此外，膜转运途径中预测的微生物群落代谢功能的增加提供了关键证据，表明添加该益生菌对仔猪可能有益，因为这些途径对于为后代提供营养和免疫至关重要。本研究突出了母猪乳汁中微生物群落组成的重要性，以及添加地衣芽孢杆菌作为改善仔猪健康和发育手段的潜力。
