Wen Xiaokun, Xue Zhibo, Wang Kexin, Li Jun, Ding Yadan, Wang Guorui, Xu Haiyang, Hong Xia
Key Laboratory of UV-Emitting Materials and Technology (Northeast Normal University), Ministry of Education, Changchun, 130024, P. R. China.
College of Physics, Liaoning University, Shenyang, 110036, P. R. China.
Mikrochim Acta. 2025 Jan 2;192(1):49. doi: 10.1007/s00604-024-06920-1.
A novel dual-mode detection method for microRNA-21 was developed. Photoluminescent (PL) and multiphonon resonant Raman scattering (MRRS) techniques were combined by using ZnTe nanoparticles as signal probes for reliable detection. The catalytic hairpin assembly (CHA) strategy was integrated with superparamagnetic FeO nanoparticle clusters (NCs) to enhance sensitivity. A remarkable detection sensitivity was achieved, with an ultralow limit of detection (LOD) of 310 aM for PL and 460 aM for MRRS. A wide detection range spanning from 500 aM to 100 nM for PL and 500 aM to 10 nM for MRRS was demonstrated, showcasing the versatility and efficacy of the method. Comparing to current methods and our previous work, both sensitivity and detection range showed significant advancements. The consistency between the detection results of PL and MRRS modes highlights the reliability and robustness of our method, offering compelling internal validation. This work not only opens new avenues for achieving sensitive and accurate detection of miRNAs, but also shows significant promise for advancing diagnostic applications in disease management.
开发了一种用于检测微小RNA-21的新型双模式检测方法。通过使用碲化锌纳米颗粒作为信号探针，将光致发光（PL）技术与多声子共振拉曼散射（MRRS）技术相结合，以实现可靠检测。将催化发夹组装（CHA）策略与超顺磁性氧化亚铁纳米颗粒簇（NCs）相结合，以提高检测灵敏度。实现了显著的检测灵敏度，PL的超低检测限（LOD）为310 aM，MRRS的超低检测限为460 aM。展示了PL的检测范围为500 aM至100 nM，MRRS的检测范围为500 aM至10 nM，体现了该方法的通用性和有效性。与当前方法和我们之前的工作相比，灵敏度和检测范围均有显著提升。PL和MRRS模式检测结果之间的一致性突出了我们方法的可靠性和稳健性，提供了令人信服的内部验证。这项工作不仅为实现对微小RNA的灵敏和准确检测开辟了新途径，而且在推进疾病管理中的诊断应用方面也显示出巨大潜力。
