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海榄雌叶提取物对鲤鱼养殖中病原菌的生物活性。

Biopotency of Avicennia marina leaf extracts against pathogenic bacteria in carp culture.

作者信息

Sravya M V N, Sandeep T Rahul, Beulah G, Sampath Kumar N S, Simhachalam G

机构信息

Department of Zoology and Aquaculture, Acharya Nagarjuna University, Guntur, 522510, Andhra Pradesh, India.

Department of Biotechnology, Vignan's Foundation for Science, Technology and Research, Vadlamudi, 522213, Andhra Pradesh, India.

出版信息

AMB Express. 2025 Jan 2;15(1):2. doi: 10.1186/s13568-024-01791-4.

Abstract

Disease emergence has become a main limiting factor in aquaculture. The massive application of antibiotics as disease therapy has been resulting in the adverse effects of environment, host and consumers. Ethyl acetate leaf extract of A.marina has recorded high biological activity. Crude extract has showed the antibacterial activity of range 3.2 ± 0.8 mm against B.subtilis to 5.7 ± 0.7 mm against A.hydrophila, DPPH; 15.9 ± 0.7 AAE µg/ml to 79.4 ± 1.0 AAE µg/ml and FRAP; 6.4 ± 1.3 AAE µg/ml to 127.3 ± 1.3 AAE µg/ml. The mass spectral results revealed the presence of thioacetic acid, methyl thio ethane, 1-Fluoro-2-propanone, Isopropanethiol. CAT and SOD levels of ethyl acetate extract treated fingerlings was: crude; 15.5 ± 1.0 units/mg of protein and 13 ± 0.9 units/mg of protein, purified 18.3 ± 0.5 units/mg of protein and 16.9 ± 1.1 units/mg of protein.

摘要

疾病的出现已成为水产养殖的主要限制因素。大量使用抗生素治疗疾病已对环境、宿主和消费者产生不利影响。滨海艾的乙酸乙酯叶提取物具有很高的生物活性。粗提物对枯草芽孢杆菌的抑菌活性范围为3.2±0.8毫米,对嗜水气单胞菌的抑菌活性为5.7±0.7毫米,DPPH;15.9±0.7 AAE微克/毫升至79.4±1.0 AAE微克/毫升,FRAP;6.4±1.3 AAE微克/毫升至127.3±1.3 AAE微克/毫升。质谱结果显示存在硫代乙酸、甲硫乙烷、1-氟-2-丙酮、异丙硫醇。乙酸乙酯提取物处理的鱼种的CAT和SOD水平为:粗提物;15.5±1.0单位/毫克蛋白质和13±0.9单位/毫克蛋白质,纯化后为18.3±0.5单位/毫克蛋白质和16.9±1.1单位/毫克蛋白质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b278/11695665/94c47ac0f7bf/13568_2024_1791_Fig1_HTML.jpg

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