Sravya M V N, Simhachalam G, Kumar N S Sampath, Govindarao K, Sandeep T Rahul, Divya D
Department of Zoology and Aquaculture, Acharya Nagarjuna University, Guntur, Andhra Pradesh, 522510, India.
Department of Biotechnology, Vignan's Foundation for Science, Technology and Research, Vadlamudi, Andhra Pradesh, 522213, India.
AMB Express. 2023 Aug 20;13(1):86. doi: 10.1186/s13568-023-01595-y.
Antibiotic resistance has become one of the inevitable barrier in aquaculture disease management. Herbal drugs has evolved to be the novel ways of combating drug resistant pathogens. In the current investigation, leaf extracts of mangrove plant, Acanthus ilicifolius were assessed for in vitro studies, among the selected four extracts, methanol extract has expressed highest antibacterial activity against P .aeruginosa (4 ± 0.3 mm), A. hydrophila (5.9 ± 0.5 mm), S. aureus (3.5 ± 0.7 mm) and B. subtilis (2.9 ± 0.5 mm) and antioxidant activity, DPPH (81.3 ± 1.0 AAEµg/ml) and FRAP (139.1 ± 1.5 AAEµg/ml).TPC and TFC were higher in the methanolic extract and has exhibited positive correlation with both DPPH and FRAP assays. Considering the in vitro efficiency, methanol extract was purified successively by column and thin layer chromatography and characterisation by GC-MS unveiled the presence of 2-Propanethiol, Trimethylphosphine, Pentanoyl chloride, Dimethylhydroxymethylphosphine and Propanedinitrile, ethylidene. A. hydrophila infected L. rohita fingerlings has survival percentage 81% and 94% in extract treated groups over 0% in negative control and 71% in positive control.
抗生素耐药性已成为水产养殖疾病管理中不可避免的障碍之一。草药已发展成为对抗耐药病原体的新方法。在当前的研究中，对红树林植物老鼠簕的叶提取物进行了体外研究，在所选择的四种提取物中，甲醇提取物对铜绿假单胞菌（4±0.3毫米）、嗜水气单胞菌（5.9±0.5毫米）、金黄色葡萄球菌（3.5±0.7毫米）和枯草芽孢杆菌（2.9±0.5毫米）表现出最高的抗菌活性以及抗氧化活性，DPPH（81.3±1.0抗坏血酸当量微克/毫升）和FRAP（139.1±1.5抗坏血酸当量微克/毫升）。总酚含量（TPC）和总黄酮含量（TFC）在甲醇提取物中较高，并且与DPPH和FRAP测定均呈正相关。考虑到体外效率，甲醇提取物通过柱色谱和薄层色谱依次纯化，气相色谱-质谱联用（GC-MS）表征揭示了2-丙硫醇、三甲基膦、戊酰氯、二甲基羟甲基膦和亚乙基丙二腈的存在。在提取物处理组中，感染嗜水气单胞菌的露斯塔野鲮鱼种的存活率为81%和94%，而阴性对照组为0%，阳性对照组为71%。
